Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.2K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.2K
Meiosis II02:02

Meiosis II

45.7K
Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
45.7K
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

3.6K
At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
3.6K
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

2.9K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
2.9K
Crossing Over01:34

Crossing Over

146.8K
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process...
146.8K
Meiosis I03:09

Meiosis I

40.6K
Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
40.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Association Between Female Reproductive Factors and Laryngopharyngeal Reflux: A National Population-Based Study.

Journal of clinical medicine·2026
Same author

Association of Early Supplemental Parenteral Nutrition with Clinical Outcomes in Patients with Traumatic Brain Injury.

Journal of Korean Neurosurgical Society·2026
Same author

Effect of an annotation-free artificial intelligence system for simultaneous detection and diagnosis on breast ultrasonography: a multireader, multicase study across diverse healthcare professionals.

Ultrasonography (Seoul, Korea)·2026
Same author

The STA1-DOT2 interaction promotes nuclear speckle formation and splicing robustness in growth and heat stress responses.

The Plant cell·2026
Same author

Apomeiosis circumvents sterility in inter-subspecific hybrids of rice.

The Plant journal : for cell and molecular biology·2026
Same author

Predictions of Oncotype DX<sup>®</sup> High-Risk Classification Using Magnetic Resonance Imaging-Based Intratumoral Heterogeneity.

Bioengineering (Basel, Switzerland)·2026

Related Experiment Video

Updated: Jul 2, 2025

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

2.0K

Control of meiotic crossover interference by a proteolytic chaperone network.

Heejin Kim1, Jaeil Kim1, Namil Son1

  • 1Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.

Nature Plants
|February 21, 2024
PubMed
Summary

Researchers discovered that the J3 chaperone, linked to HSP40, regulates meiotic recombination by controlling HEI10 protein levels. This finding sheds light on how crossovers are distributed during meiosis, impacting genetic diversity.

More Related Videos

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

11.7K
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

1.8K

Related Experiment Videos

Last Updated: Jul 2, 2025

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

2.0K
Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing
09:16

Meiotic Spindle Assessment in Mouse Oocytes by siRNA-mediated Silencing

Published on: October 11, 2015

11.7K
Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae
07:48

Use of Time-Lapse Microscopy and Stage-Specific Nuclear Depletion of Proteins to Study Meiosis in S. cerevisiae

Published on: October 11, 2022

1.8K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Meiosis is crucial for sexual reproduction, generating genetic diversity through homologous chromosome crossovers.
  • Crossover distribution is tightly regulated by interference, ensuring at least one exchange per chromosome pair.
  • The E3 ligase HEI10 is implicated in crossover site selection in Arabidopsis, but its regulation remains unclear.

Purpose of the Study:

  • To identify genetic factors controlling HEI10 dynamics and meiotic crossover distribution in Arabidopsis.
  • To elucidate the molecular mechanisms underlying crossover interference and recombination landscape regulation.

Main Methods:

  • Forward genetic screen in Arabidopsis to identify mutants affecting crossover rates.
  • Characterization of the high crossover rate3 (hcr3) mutant and identification of the HCR3 gene.
  • Biochemical and genetic analyses to determine the function of HCR3/J3 and its interaction with HSP70 and HEI10.

Main Results:

  • Identification of hcr3, a dominant-negative mutant that reduces crossover interference and increases genome-wide crossovers.
  • HCR3 encodes J3, an HSP40 co-chaperone that targets protein aggregates and condensates for degradation.
  • The J3-HSP70 chaperone network facilitates HEI10 proteolysis, thereby regulating meiotic recombination and interference.

Conclusions:

  • The HSP40/J3-HSP70 chaperone system plays a novel role in regulating meiotic recombination dynamics.
  • Control of HEI10 proteolysis by J3-HSP70 is a key mechanism for managing crossover interference and distribution.
  • These findings reveal a new layer of regulation in the meiotic recombination landscape, impacting genetic variation.