Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

51.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
51.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.0K
Homologous Recombination02:31

Homologous Recombination

50.5K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.5K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.0K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequence-Dependent Modification of Bamboo Shoot Dietary Fiber Through Enzymatic Hydrolysis and <i>Lactobacillus plantarum</i> Fermentation.

Foods (Basel, Switzerland)·2026
Same author

3‑Methylthiopropionic Acid Kills Carbapenem-Resistant <i>Klebsiella pneumoniae</i> by Disrupting Membrane Integrity and Bioenergetics.

JACS Au·2026
Same author

NIR-II Light-Modulated Smart Drug Delivery System Utilizing Drug-Gated Nanocomposite Hydrogel for Boosting Anticancer Efficacy.

Polymer science & technology (Washington, D.C.)·2026
Same author

One-shot quantification of 313 PPCPs and pesticides in bovine milk by modified-QuEChERS-UHPLC-MS/MS.

Food chemistry·2026
Same author

Characterization and Tracing of Soilborne Lead-Containing Nanoparticles by Single-Particle Inductively Coupled Plasma Mass Spectrometry.

Analytical chemistry·2026
Same author

IS110 transposon utilizes two mechanistically distinct RNA-guided transposition pathways.

Molecular cell·2026

Related Experiment Video

Updated: Jul 2, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.7K

Recent advances in CRISPR-Cas9-based genome insertion technologies.

Xinwen Chen1,2, Jingjing Du1,2, Shaowei Yun1,2

  • 1State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.

Molecular Therapy. Nucleic Acids
|February 21, 2024
PubMed
Summary

This review explores programmable genome insertion techniques, including CRISPR-based methods. It details the pros and cons of each tool to guide researchers in basic research and therapeutic applications.

Keywords:
CRISPR-associated transposonDSB-dependent knock-inMT: RNA/DNA editingcell and gene therapyprime editingprogrammable genome insertion

More Related Videos

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

14.9K

Related Experiment Videos

Last Updated: Jul 2, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

21.7K
Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology
10:07

Construction of Homozygous Mutants of Migratory Locust Using CRISPR/Cas9 Technology

Published on: March 16, 2022

2.1K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

14.9K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Programmable genome insertion is crucial for biological research and therapeutic development.
  • CRISPR-based technologies have significantly advanced genome editing capabilities.
  • Existing methods often rely on cellular DNA repair pathways like HDR, NHEJ, and MMEJ.

Purpose of the Study:

  • To review recent advancements in programmable genome insertion techniques.
  • To analyze the advantages and disadvantages of various genome insertion tools.
  • To identify future directions for improving genome editing technologies.

Main Methods:

  • Summary of recent literature on programmable genome insertion strategies.
  • Comparative analysis of techniques such as prime editing, integrase-mediated insertion, and CRISPR-associated transposons.
  • Discussion of efficiency, targeting scope, and specificity of different methods.

Main Results:

  • CRISPR-based genome insertion strategies are rapidly evolving.
  • New tools like prime editing and CRISPR-associated transposons expand the possibilities for precise genome modification.
  • Each technique presents unique strengths and limitations impacting its application.

Conclusions:

  • Informed selection of genome insertion tools is essential for successful research and therapeutic applications.
  • Ongoing efforts focus on enhancing editing efficiency, specificity, and targeting range.
  • Future improvements hold promise for advancing both fundamental biological understanding and clinical treatments.