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Probing efficient microbial CO2 utilisation through metabolic and process modelling.

Philip J Gorter de Vries1, Viviënne Mol1, Nikolaus Sonnenschein2

  • 1The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.

Microbial Biotechnology
|February 21, 2024
PubMed
Summary
This summary is machine-generated.

Acetogenic gas fermentation can be optimized for waste gas upcycling. Modeling shows higher temperatures and specific H2:CO2 ratios improve yields, but expanding product range beyond acetate remains challenging.

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Area of Science:

  • Biotechnology
  • Chemical Engineering
  • Environmental Science

Background:

  • Acetogenic gas fermentation offers a promising route for upcycling carbon-rich waste gases.
  • Current limitations in product range, yield, rate, and titre hinder economic viability.

Purpose of the Study:

  • To optimize acetogenic gas fermentation processes through integrated process and metabolic modeling.
  • To explore conditions and strategies for expanding the product portfolio beyond acetate.

Main Methods:

  • Paired process modeling and host-agnostic metabolic modeling were employed.
  • Simulations were conducted for an industrial-scale bubble column reactor.
  • Product volatility analysis and metabolic pathway modeling were utilized.

Main Results:

  • Increased temperatures enhance gas transfer rates, especially for H2.
  • An optimal feed composition of 9:1 mol H2 to mol CO2 was predicted.
  • Thermophilic metabolism favors catabolic products; expanding product range is metabolically unfavorable compared to acetate/ethanol.

Conclusions:

  • Process optimization can improve acetogenic gas fermentation efficiency.
  • In-situ recovery of volatile products like acetone is feasible but challenging.
  • Alternative strategies are needed to overcome metabolic limitations for producing diverse carbon-negative chemicals via acetogenic CO2 fixation.