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Heparinized Acellular Hydrogels for Magnetically Induced Wound Healing Applications
Filipa Pires1,2, João Carlos Silva3,4, Frederico Castelo Ferreira3,4
1Instituto de Telecomunicações, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
ACS Applied Materials & Interfaces
|February 21, 2024
Summary
Magnetic hydrogels with heparin and mesenchymal stem/stromal cells (MSCs) enhance wound healing by promoting angiogenesis. Heparinized scaffolds retain growth factors, improving wound contraction and cell migration for tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Angiogenesis control is crucial for therapeutic strategies in wound healing and cancer.
- Mesenchymal stem/stromal cells (MSCs) possess anti-inflammatory and angiogenic properties beneficial for tissue repair.
Purpose of the Study:
- To investigate the effect of heparin and magnetic nanoparticles (MNPs) in gelatin scaffolds on MSCs behavior under magnetic field (MF).
- To optimize hydrogel properties and MSC secretome for enhanced tissue healing and angiogenesis.
Main Methods:
- MSCs were seeded on gelatin scaffolds functionalized with MNPs and varying heparin concentrations.
- Scaffolds were exposed to a static 0.08 T magnetic field.
- Hydrogel mechanical properties, MSC morphology, proliferation, and secretome were analyzed.
- Wound contraction and cell migration rates were assessed in vitro.
Main Results:
- MNPs and heparin influenced hydrogel swelling and MSC proliferation rates.
- MF guided MSC alignment and upregulated pro-angiogenic gene/protein expression.
- High heparin concentrations (10 μg/cm³) hindered growth factor diffusion.
- Heparinized magnetic hydrogels significantly improved wound contraction (55.8%) and cell migration (49.4%) compared to non-heparinized ones.
Conclusions:
- Heparinized magnetic hydrogels effectively retain angiogenic factors released by magnetically stimulated MSCs.
- These optimized scaffolds show superior performance in promoting wound healing and tissue regeneration.
- The developed system holds promise for treating various tissue damages, including bone defects, skin wounds, and cardiovascular diseases.
