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The Functional Circular RNA Screening via RfxCas13d/BSJ-gRNA System
Hao Wu1, Ling-Ling Chen2,3,4
1Key Laboratory of RNA Science and Engineering, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, China. wuhao2021@sibcb.ac.cn.
Methods in Molecular Biology (Clifton, N.J.)
|February 21, 2024
Summary
Researchers developed a new CRISPR-Cas13 method for large-scale functional screening of circular RNAs (circRNAs). This tool helps identify circRNAs impacting cell growth without affecting linear RNAs.
Area of Science:
- Molecular Biology
- RNA Biology
- Genomics
Background:
- Circular RNAs (circRNAs) are formed by pre-mRNA back-splicing, but their functions remain largely unexplored.
- Distinguishing circRNAs from their cognate linear mRNAs for functional studies is challenging due to sequence homology.
Purpose of the Study:
- To develop a method for large-scale functional screening of circRNAs.
- To enable the identification of circRNAs that regulate cell growth under various conditions.
Main Methods:
- Utilized the RNA-guided, RNA-targeting CRISPR-Cas13 (RfxCas13d) system for programmable circRNA screening.
- Applied the method in vivo and in cell for functional analysis.
Main Results:
- Demonstrated a method for high-throughput circRNA functional screening.
- Enabled the exploration of circRNA roles in cell growth regulation.
Conclusions:
- The developed CRISPR-Cas13 based method allows for efficient and specific functional screening of circRNAs.
- This approach facilitates the discovery of novel circRNA functions in cellular processes.
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