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Updated: Jul 2, 2025

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Identification of RNAs Engaged in Direct RNA-RNA Interaction with a Long Non-Coding RNA

Published on: July 9, 2021

Native Circular RNA Pulldown Method to Simultaneously Profile RNA and Protein Interactions.

Marta M Gabryelska¹, Stuart T Webb¹, He Lin¹

¹Flinders Health and Medical Research Institute (FHMRI), College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.

Methods in Molecular Biology (Clifton, N.J.)

|February 21, 2024

Summary

This summary is machine-generated.

This study introduces a new method to isolate the circular RNA interactome in cells. This technique helps identify crucial RNA-RNA and RNA-protein interactions for understanding circular RNA functions.

Keywords:

Interactome Pulldown RNA-binding proteins RNA–RNA interactions circRNA circRNA–RNA circRNA–protein circular RNA

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Last Updated: Jul 2, 2025

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Published on: July 9, 2021

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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Area of Science:

Molecular Biology
Genomics
Biochemistry

Background:

Circular RNAs (circRNAs) are prevalent, non-coding RNA transcripts with cell-, tissue-, and disease-specific expression patterns.
CircRNAs originate from pre-mRNA via non-canonical splicing (back-splicing), forming covalently-closed structures with a unique backsplice junction (BSJ).
These molecules play significant roles in cellular processes through interactions with proteins and other RNAs.

Purpose of the Study:

To develop and optimize a strategy for simultaneously purifying the circRNA interactome within eukaryotic cells.
To enable the identification of both circRNA-RNA and circRNA-protein interactions.
To elucidate the molecular mechanisms underlying circRNA function.

Main Methods:

Development of an optimized protocol for circRNA interactome purification.
Application of the method in eukaryotic cell systems.
Simultaneous identification of interacting RNA and protein partners.

Main Results:

Successful simultaneous purification of the circRNA interactome.
Identification of specific circRNA-RNA interactions.
Identification of specific circRNA-protein interactions.

Conclusions:

The presented strategy is effective for comprehensive circRNA interactome analysis.
This method facilitates the discovery of novel circRNA functions and regulatory mechanisms.
Understanding circRNA interactions is key to deciphering their roles in various biological contexts.