Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of hyperlipidic diet-induced obesity on placental transcriptome and fetal development: FTO-related biological processes.

Placenta·2026
Same author

Monocyte-red blood cell crosstalk supports clearance and heme metabolism in sickle cell anemia.

Frontiers in immunology·2025
Same author

GWAS for primary angle-closure glaucoma identifies loci related to ocular biometry and morphology.

Nature communications·2025
Same author

Circulating Monocytes Contribute to Erythrocyte Clearance in Polycythemia Vera.

International journal of molecular sciences·2025
Same author

Iron overload is not the same everywhere: Particularities of iron-metabolism gene mutations in Brazil and a proposal for the investigation and management of iron overload in this population.

Hematology, transfusion and cell therapy·2025
Same author

Updated classification of epileptic seizures: Position paper of the International League Against Epilepsy.

Epilepsia·2025

Related Experiment Video

Updated: Jul 2, 2025

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

21.9K

Comparative transcriptomic analysis of circulating endothelial cells in sickle cell stroke.

Júlia Nicoliello Pereira de Castro1, Sueli Matilde da Silva Costa1, Ana Carolina Lima Camargo1

  • 1Laboratory of Human Genetics, Center for Molecular Biology and Genetic Engineering-CBMEG, Universidade Estadual de Campinas-UNICAMP, Campinas, São Paulo, 13083-875, Brazil.

Annals of Hematology
|February 22, 2024
PubMed
Summary

Sickle cell anemia (SCA) patients with ischemic stroke (IS) show altered endothelial cells. Gene expression analysis reveals persistent angiogenic processes, offering potential targets for stroke recovery in SCA.

Keywords:
Endothelial colony-forming cellsIschemic strokeRNA-SeqSickle cell anemia

More Related Videos

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.2K
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
11:08

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

16.0K

Related Experiment Videos

Last Updated: Jul 2, 2025

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells
18:30

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

Published on: February 13, 2013

21.9K
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
09:12

Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Published on: October 4, 2021

3.2K
Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases
11:08

Endothelialized Microfluidics for Studying Microvascular Interactions in Hematologic Diseases

Published on: June 22, 2012

16.0K

Area of Science:

  • Vascular biology
  • Hematology
  • Genomics

Background:

  • Ischemic stroke (IS) is a major complication of sickle cell anemia (SCA), contributing to significant mortality.
  • Vasculopathy in SCA involves rheological changes, altered cell adhesion, inflammation, and endothelial dysfunction.
  • The endothelium plays a critical role in ischemia-induced repair and neovascularization.

Purpose of the Study:

  • To conduct a comparative transcriptomic analysis of endothelial colony-forming cells (ECFCs) from SCA patients with and without IS.
  • To identify differentially expressed genes (DEGs) and understand their biological relevance in the context of IS in SCA.
  • To explore potential molecular targets for improving stroke outcomes in SCA.

Main Methods:

  • Comparative transcriptomic analysis of ECFCs from SCA patients (with and without IS) and controls.
  • Functional enrichment analysis of DEGs.
  • Protein-protein interaction (PPI) network construction and in silico prediction of regulatory factors.

Main Results:

  • Identified 2469 DEGs between ECFCs from SCA patients with and without IS.
  • DEGs are associated with key endothelial cell functions including proliferation, migration, angiogenesis, and defense response.
  • Genes like AKT1, E2F1, CDCA5, EGFL7, HRAS, IRF3, and TGFB1 were highlighted for their roles in these pathways.
  • Evidence suggests a persistent angiogenic process in ECFCs from IS patients, even long after the event.

Conclusions:

  • The study provides novel insights into the molecular mechanisms underlying IS in SCA by analyzing ECFC gene expression.
  • Identified DEGs and pathways offer potential molecular targets for therapeutic interventions aimed at stroke outcome improvement in SCA.
  • The findings highlight the persistent angiogenic activity in ECFCs from IS patients, contributing to understanding endothelial cell roles in stroke recovery.