Related Experiment Video
Updated: Jul 2, 2025
Stimulation of Notch Signaling in Mouse Osteoclast Precursors
Published on: February 28, 2017
[Resveratrol Inhibits T-acute Lymphoblastic Leukemia in Mice by Regulating Notch1 Signaling Pathway]
Xiao-Fei Li1, Fang Cui2, Fei Liu1
1Department of Clinical Laboratory Examination, The Second Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei Province, China.
Objective:
To observe the effect of resveratrol (Res) on T-acute lymphoblastic leukemia (T-ALL) mice, and further explore its mechanism on Notch1 signaling pathway.
Methods:
Twenty-five 6-8 weeks old female C57BL/6 mice were randomly divided into control group, T-ALL group and Res group. Res group was further divided into low-Res, middle-Res and high-Res group. The percentage of leukemia cells in peripheral blood and spleen cell suspension were detected by flow cytometry and Wright-Giemsa staining, pathological morphology of spleen and bone marrow tissues were observed by HE staining, the expression levels of Notch1, Hes-1, c-Myc, miR-19b and PTEN mRNA in spleen tissue were detected by RT-qPCR, and the protein levels of Notch1, Hes-1, c-Myc, p-PTEN and PTEN were detected by Western blot.
Results:
Compared with control group, the leukemia cells in peripheral blood of mice in T-ALL group were markedly increased, accompanied by diffuse infiltration of leukemia cells in spleen and bone marrow tissues, the mRNA levels of Notch1, Hes-1, c-Myc, miR-19b and the protein levels of Notch1, Hes-1, c-Myc were increased (P <0.01), while the expression of PTEN mRNA and protein were significantly decreased in the spleen tissue of T-ALL mice (P <0.01). The above indicators in the H-Res group were reversed compared with T-ALL group after administration of resveratrol.
Conclusion:
Resveratrol may play a role in anti T-ALL by inhibiting Notch1 signaling pathway in mice.
Insights
Resveratrol demonstrated anti-leukemia effects in T-acute lymphoblastic leukemia (T-ALL) mice by inhibiting the Notch1 signaling pathway. This natural compound reversed key indicators of leukemia progression, offering potential therapeutic benefits.
Area of Science:
- Biochemistry
- Molecular Biology
- Oncology
Context:
- T-acute lymphoblastic leukemia (T-ALL) is an aggressive hematological malignancy.
- The Notch1 signaling pathway is frequently implicated in T-ALL pathogenesis.
- Resveratrol, a natural polyphenol, has shown potential anti-cancer properties.
Purpose:
- To investigate the therapeutic effect of resveratrol on T-ALL in a mouse model.
- To elucidate the underlying mechanism of resveratrol's action, focusing on the Notch1 signaling pathway.
Summary:
- Resveratrol administration significantly reduced leukemia cell percentages in peripheral blood and spleen.
- Key markers of T-ALL progression, including Notch1, Hes-1, c-Myc, and miR-19b, were downregulated by resveratrol.
- Conversely, PTEN expression was upregulated by resveratrol, indicating pathway modulation.
Impact:
- Resveratrol exhibits anti-leukemic activity against T-ALL in vivo.
- Inhibition of the Notch1 signaling pathway is a potential mechanism for resveratrol's anti-TALL effects.
- These findings suggest resveratrol as a promising candidate for T-ALL therapy.
Related Concept Videos
03:14Notch Signaling Pathway
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
01:12Role Of Notch Signalling In Intestinal Stem Cell Renewal
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
01:15Receptor Downregulation in MVBs
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
01:20The Retinoblastoma Gene
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...