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Fabrication Method for Shape-Controlled 3D Tissue Using High-Porosity Porous Structure.

Hidetaka Ueno^1,2,3, Shohei Yamamura³

¹Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, 1-5-1 Minatojima-minamimachi, Chuo-ku, Kobe-city 650-0047, Hyogo, Japan.

Bioengineering (Basel, Switzerland)

|February 23, 2024

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Researchers developed a novel method to create shape-controlled 3D tissues without non-natural materials. This technique uses a flexible high-porosity porous structure (HPPS) for efficient fabrication and maintains high cell viability.

Area of Science:

Biomaterials Engineering
Tissue Engineering
Regenerative Medicine

Background:

Shape-controlled 3D tissues are crucial for regenerative medicine, drug discovery, and biological robotics.
Existing methods using scaffolds or 3D bioprinting face challenges in controlling shape without residual materials and ensuring efficient fabrication.
A need exists for methods that produce natural, shape-defined 3D tissues free from artificial components.

Purpose of the Study:

To propose and validate a novel method for fabricating shape-controlled 3D tissues free of non-natural materials.
To demonstrate the efficiency and biocompatibility of the proposed fabrication technique.
To enable the development of more physiologically relevant 3D tissue models.

Main Methods:

Fabrication of a flexible high-porosity porous structure (HPPS) with specific micro-dimensions (e.g., 14.87 ± 1.83 μm pores, 69.06 ± 3.30% porosity).

Keywords:

3D tissue SU-8 high-porosity porous structure micromesh tissue engineering

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Culturing U-87 human glioma cells within an I-shaped HPPS microchamber for 48 hours.

Releasing the fabricated 3D tissue rapidly without chemical treatments, preserving its shape and cell integrity.

Main Results:

The HPPS demonstrated controlled micro-architectural properties suitable for tissue culture.
Shape-controlled 3D tissues were successfully fabricated and released within seconds, maintaining their defined I-shape.
Cell viability within the released 3D tissues exceeded 90%, indicating minimal stress and high biocompatibility.

Conclusions:

The proposed HPPS-based method offers an efficient and non-invasive approach for fabricating shape-controlled 3D tissues.
This technique successfully produces 3D tissues free of non-natural materials, enhancing their suitability for biological applications.
The method holds significant promise for advancing regenerative medicine and drug discovery by providing more naturalistic tissue models.