Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Color-tunable microfluidic synthesis of FITC/RITC-functionalized POSS hybrid nanoparticles and <i>in vitro</i> biocompatibility assessment.

Journal of materials chemistry. B·2026
Same author

Magneto-electrochemical biosensing for pathogen detection using nuclease-responsive nanohybrids.

Mikrochimica acta·2026
Same author

Multifunctional POSS-based nanoparticles functionalized with silver, SPIONs, and rhamnolipid for antibacterial applications.

Biomaterials advances·2026
Same author

Nanoencapsulation of hydroxytyrosol extract of fermented olive leaf brine using proniosomes.

Journal of the science of food and agriculture·2025
Same author

Assessment of Anticancer Effects of Aloe vera on 3D Liver Tumor Spheroids in a Microfluidic Platform.

Biotechnology and bioengineering·2025
Same author

Microfluidic rapid isolation and electrochemical detection of S. pneumonia via aptamer-decorated surfaces.

Analytica chimica acta·2025

Related Experiment Video

Updated: Jul 2, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

10.6K

Biosensor for ATP detection via aptamer-modified PDA@POSS nanoparticles synthesized in a microfluidic reactor.

Güneş Kibar1,2,3, O Berkay Şahinoğlu3,4, Betül Kılınçlı3,5

  • 1Dept. Materials Sci. & Eng., A.T. Adana Sci. & Tech. Uni., Adana, 01250, Turkey.

Mikrochimica Acta
|February 23, 2024
PubMed
Summary

Researchers developed aptamer-functionalized polyhedral oligomeric silsesquioxane (POSS) nanoparticles for detecting adenosine triphosphate (ATP). Microfluidic synthesis enabled efficient nanoparticle production for advanced biosensor applications.

Keywords:
ATP detectionAptasensorBiosensorMicrofluidicsPolyhedral oligomeric silsesquioxane (POSS)

More Related Videos

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

869
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.4K

Related Experiment Videos

Last Updated: Jul 2, 2025

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

10.6K
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

869
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

1.4K

Area of Science:

  • Nanotechnology
  • Biomaterials Science
  • Analytical Chemistry

Background:

  • Developing sensitive and specific detection methods for biomolecules like adenosine triphosphate (ATP) is crucial for various applications.
  • Polyhedral oligomeric silsesquioxane (POSS) nanoparticles offer unique properties for nanomaterial-based applications.
  • Aptamers are valuable recognition elements in biosensing due to their specificity and stability.

Purpose of the Study:

  • To synthesize aptamer-functionalized POSS nanoparticles using a microfluidic reactor for adenosine triphosphate (ATP) detection.
  • To evaluate the performance of these aptamer-modified POSS nanoparticles in affinity-based biosensor applications.
  • To demonstrate the feasibility of using microfluidic synthesis for creating functionalized POSS nanoparticles for biorecognition.

Main Methods:

  • POSS nanoparticles were synthesized via thermal polymerization in a one-step, continuous flow microfluidic reactor designed to prevent clogging.
  • The surface of POSS nanoparticles was modified using polydopamine.
  • ATP-specific aptamers were successfully attached to the polydopamine-coated POSS nanoparticles.
  • The affinity of the aptamer-modified POSS nanoparticles for ATP was determined.

Main Results:

  • A continuous flow microfluidic reactor was successfully employed for the one-step synthesis of POSS nanoparticles.
  • Biomimetic surface modification with polydopamine allowed for efficient aptamer conjugation.
  • The aptamer-functionalized POSS nanoparticles demonstrated successful capture of ATP molecules.
  • An affinity constant of 46.5 µM was determined for ATP binding to the aptamer-modified POSS nanoparticles.

Conclusions:

  • Microfluidic synthesis provides an efficient method for producing POSS nanoparticles for biosensor development.
  • Aptamer-functionalized POSS nanoparticles are effective for adenosine triphosphate (ATP) detection.
  • This study highlights the potential of POSS nanoparticles in advanced biorecognition and sample preparation for biosensing applications.