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Updated: Aug 2, 2026
08:55
Isolation and Characterization of Primary Rat Valve Interstitial Cells: A New Model to Study Aortic Valve Calcification
Published on: November 20, 2017
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Transcriptomic Analysis of Mineralized Adipose-Derived Stem Cell Tissues for Calcific Valve Disease Modelling
Alyssa Brodeur1,2, Vincent Roy1,2, Lydia Touzel-Deschênes1,2
1Department of Surgery, Faculty of Medicine, Université Laval, Quebec City, QC G1V 5C3, Canada.
International Journal of Molecular Sciences
|February 24, 2024
Summary
Tissue-engineered adipose-derived stromal/stem cells mimic calcific aortic valve disease (CAVD) in vitro. Extracellular matrix gene expression in these models shows similarities to CAVD, highlighting ECM
Area of Science:
- Biomedical Engineering
- Cardiovascular Research
- Stem Cell Biology
Background:
- Calcific aortic valve disease (CAVD) involves aortic valve fibrosis and mineralization, leading to stenosis and heart failure.
- Human valve interstitial cells (VICs) are difficult to obtain for in vitro studies of CAVD.
- Adipose-derived stromal/stem cells (ASCs) offer a patient-specific, readily available alternative for disease modeling.
Purpose of the Study:
- To perform transcriptomic analysis of tissue-engineered, scaffold-free ASC-embedded mineralized sheets.
- To investigate the role of extracellular matrix (ECM) in ASC-driven mineralization.
- To compare gene expression profiles with human CAVD datasets.
Main Methods:
- Bulk RNA sequencing of tissue-engineered ASC-embedded mineralized sheets.
- Bioinformatic and gene set enrichment analyses.
- Comparison with publicly available CAVD gene expression data.
Main Results:
- Up-regulation of ECM organization genes observed in engineered tissues.
- Similarities in cardiovascular and ECM-related gene expression between engineered tissues and CAVD patients.
- ECM promotes RUNX2 activation, a key factor in CAVD.
Conclusions:
- Tissue-engineered ASC sheets provide a viable in vitro model for CAVD research.
- ECM plays a significant role in CAVD pathogenesis.
- This model may aid in developing novel aortic valve replacement therapies.
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