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Micro Rna 182-3-P, 519-D-5p, 378-3p As Non-Invasive Predictors Of Preeclampsia
Zaima Ali1, Uzma Zafar1, Ambreen Tauseef2
1Department of Physiology, Lahore Medical & Dental College, Lahore.
Background:
MicroRNAs (miRNAs) are an emerging field of interest in many diseases. Some of the miRNAs have been reported to be expressed differentially in diseased states of pregnancy. The current study was designed to measure and compare the levels of microRNA 182-3-p, 519-d-5p, and 378-3p and it was hypothesized that the microRNA 182-3-p, 519-d-5p, and 378-3p can be used as a non-invasive predictor of preeclampsia.
Methods:
Expression level of the miRNAs 182-3-p, 519-d-5p, and 378-3p was measured in the serum of preeclamptic and normal pregnancies by real-time PCR. Data was entered and analysed by Statistical Package for the Social Sciences 22 (SPSS).
Results:
Significantly high expression levels of MiRNA 182-3p, 519-d-5p and low levels of miR-378-3p were associated with preeclampsia (PE).
Conclusions:
The results revealed that miR-182-3p is a powerful predictor of PE with an Odds Ratio of 5.9 and can be used as a noninvasive, reliable predictor of PE to screen these patients at an early stage. Screening at early gestation with follow-up studies can emphasize the results.
Insights
MicroRNA 182-3p and 519-d-5p show high expression, while microRNA 378-3p shows low expression in preeclampsia (PE). These microRNAs can serve as non-invasive biomarkers for early PE detection.
Area of Science:
- Biochemistry
- Genetics
- Obstetrics
Background:
- MicroRNAs (miRNAs) are increasingly recognized for their role in various diseases.
- Differential expression of specific miRNAs has been observed in pregnancy-related complications.
- Preeclampsia (PE) diagnosis and monitoring can benefit from novel biomarkers.
Purpose of the Study:
- To quantify and compare serum levels of microRNA 182-3p, 519-d-5p, and 378-3p in preeclamptic and normal pregnancies.
- To investigate the potential of these miRNAs as non-invasive predictors for preeclampsia.
- To establish a diagnostic marker for early-stage preeclampsia detection.
Main Methods:
- Serum samples from preeclamptic and normal pregnancies were collected.
- Real-time PCR was utilized to measure the expression levels of miR-182-3p, miR-519-d-5p, and miR-378-3p.
- Statistical analysis was performed using SPSS version 22.
Main Results:
- Significantly elevated expression of miR-182-3p and miR-519-d-5p was observed in preeclamptic patients.
- Concurrently, significantly lower levels of miR-378-3p were detected in the serum of individuals with preeclampsia.
- miR-182-3p demonstrated a strong association with PE, indicated by an Odds Ratio of 5.9.
Conclusions:
- The study identifies miR-182-3p as a potent and reliable non-invasive biomarker for preeclampsia.
- These specific microRNAs can be utilized for early screening and diagnosis of preeclampsia.
- Further research with early gestation screening and follow-up is recommended to validate these findings.
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