Related Experiment Video
Updated: Jul 2, 2025
Biomolecular Imaging of Cellular Uptake of Nanoparticles using Multimodal Nonlinear Optical Microscopy
Published on: May 16, 2022
High contrast breast cancer biomarker semi-quantification and immunohistochemistry imaging using upconverting
Sanathana Konugolu Venkata Sekar1, Hui Ma1,2, Katarzyna Komolibus1
1Biophotonics@Tyndall, IPIC, Tyndall National Institute, Lee Maltings Complex, Dyke Parade, T12R5CP, Cork, Ireland.
Abstract:
Breast cancer is the second leading cause of cancer death in women. Current clinical treatment stratification practices open up an avenue for significant improvements, potentially through advancements in immunohistochemistry (IHC) assessments of biopsies. We report a high contrast upconverting nanoparticles (UCNP) labeling to distinguish different levels of human epidermal growth factor receptor 2 (HER2) in HER2 control pellet arrays (CPAs) and HER2-positive breast cancer tissue. A simple Fourier transform algorithm trained on CPAs was sufficient to provide a semi-quantitative HER2 assessment tool for breast cancer tissues. The UCNP labeling had a signal-to-background ratio of 40 compared to the negative control.
