Related Experiment Video
Updated: Sep 24, 2026

09:40
Detection of True IgE-expressing Mouse B Lineage Cells
Published on: December 1, 2014
Detection of eosinophils using an eosinophil peroxidase assay. Its use as an assay for eosinophil differentiation
Journal of Immunological Methods
|November 7, 1985
Abstract:
A colorimetric assay for peroxidase has been applied to the detection of eosinophils in bone marrow cultures and tissue cell suspensions. The substrate solution consists of 0.1 mM o-phenylenediamine in 0.05 M Tris-HCl buffer pH 8.0 containing 0.1% Triton and 1 mM hydrogen peroxide. The method is shown to be an easy and reproducible method of detecting eosinophils, with insignificant interference from neutrophils.

