Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

An ER-microtubule bridge: Reticulon 17 links microtubules with ER network organisation in plants.

The Plant journal : for cell and molecular biology·2026

Same author

From EM to AI: Multiscale imaging of the secretory pathway with insights from plant cells.

Journal of microscopy·2026

Same author

Phosphate starvation-induced CORNICHON HOMOLOG 5 as endoplasmic reticulum cargo receptor for PHT1 transporters in Arabidopsis.

Plant physiology·2026

Same author

Built different: ER cisternae formed by the Arabidopsis Lunapark proteins differ in ultrastructure and affect ER-Golgi transport.

The New phytologist·2026

Same author

Recent Advances and Future Perspectives in Plant Auxin Biology.

Plants (Basel, Switzerland)·2026

Same author

Amplitude- and Phase-Programmable Dual-Color Photonic Chip for High-Contrast Structured Illumination Microscopy.

ACS photonics·2026

Search research articles

Related Experiment Video

Updated: Jul 2, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.

Charlotte Pain¹, Verena Kriechbaumer¹, Alessia Candeo^2,3

¹Endomembrane Structure and Function Research Group, Department of Biological and Medical Sciences, Oxford Brookes University, Oxford, UK.

Methods in Molecular Biology (Clifton, N.J.)

|February 27, 2024

Summary

Light sheet fluorescence microscopy (LSFM) enables advanced live imaging of plant development. This study details a protocol for LSFM in Arabidopsis leaves to observe endoplasmic reticulum dynamics during heat stress.

Keywords:

Arabidopsis FIJI GFP-HDEL Light sheet fluorescence microscopy (LSFM)Photobleaching Phototoxicity

More Related Videos

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Published on: December 10, 2016

Related Experiment Videos

Last Updated: Jul 2, 2025

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Published on: February 18, 2014

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Study of Endoplasmic Reticulum and Mitochondria Interactions by In Situ Proximity Ligation Assay in Fixed Cells

Published on: December 10, 2016

Area of Science:

Plant Biology
Microscopy
Cell Biology

Background:

Light sheet fluorescence microscopy (LSFM) has revolutionized live imaging of multicellular organisms.
LSFM provides high-resolution, long-term data collection with minimal photodamage.
It is ideal for quantitative 3D time-lapse imaging in plant cell biology.

Purpose of the Study:

To present a detailed LSFM protocol for imaging Arabidopsis leaves.
To assess LSFM phototoxicity and optimize experimental setup.
To monitor endoplasmic reticulum (ER) dynamics in response to heat shock.

Main Methods:

Developing custom holders and mounting techniques for Arabidopsis leaves.
Implementing LSFM for live imaging of fluorescently tagged ER markers.
Quantifying dynamic cellular processes under controlled heat stress conditions.

Main Results:

A robust LSFM protocol for Arabidopsis leaf imaging was established.
Phototoxicity of the LSFM system was evaluated and managed.
Dynamic changes in ER structure during heat shock were successfully monitored.

Conclusions:

LSFM is a powerful technique for studying dynamic cellular processes in plants.
The presented protocol facilitates quantitative analysis of plant organelle responses to environmental stress.
This method advances our understanding of plant cell biology and stress responses.