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Updated: Jul 2, 2025

Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates
Published on: May 8, 2020
The Cdc14 phosphatase, Clp1, does not affect genome expression
Luis Lopez Maury1,2, Liping Ren3, Shaimaa Hassan1
1Department of Genetics, Evolution, and Environment, Institute of Healthy Ageing, University College London, London, UK.
Abstract:
Schizosaccharomyces pombe Clp1 is a Cdc14-family phosphatase that reverses mitotic Cdk1 phosphorylation. Despite evolutionary conservation, Clp1 's mammalian orthologs do not share this function. Rather, higher eukaryotic Cdc14 enzymes act in DNA repair, ciliogenesis, and gene regulation. To examine if Clp1 regulates gene expression, we compared the transcriptional profiles of cells lacking Clp1 function to that of wildtype. Because clp1∆ cells are sensitive to the actin depolymerizing drug, LatrunculinA, we also investigated whether a transcriptional response was involved. Our results indicate that Clp1 does not detectably affect gene expression and highlight the organism-specific functions of this conserved phosphatase family.
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