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The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
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The Cdc14 phosphatase, Clp1, does not affect genome expression.

Luis Lopez Maury1,2, Liping Ren3, Shaimaa Hassan1

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Schizosaccharomyces pombe Clp1, a phosphatase, does not regulate gene expression. This highlights conserved phosphatase family

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Area of Science:

  • Cell biology
  • Molecular genetics
  • Biochemistry

Background:

  • The Cdc14-family phosphatase Clp1 in *Schizosaccharomyces pombe* reverses mitotic Cdk1 phosphorylation.
  • Mammalian orthologs of Clp1 have different functions, including roles in DNA repair and ciliogenesis.
  • The precise role of Clp1 in gene regulation remains unclear.

Purpose of the Study:

  • To investigate whether *Schizosaccharomyces pombe* Clp1 plays a role in regulating gene expression.
  • To determine if Clp1's function is linked to the observed sensitivity of *clp1∆* cells to LatrunculinA.

Main Methods:

  • Comparative transcriptional profiling of wildtype and *clp1∆* cells.
  • Analysis of cellular responses to the actin depolymerizing drug LatrunculinA.

Main Results:

  • Clp1 was found to have no detectable effect on gene expression in *Schizosaccharomyces pombe*.
  • The sensitivity of *clp1∆* cells to LatrunculinA does not appear to be mediated by a transcriptional response.

Conclusions:

  • Clp1's function in *Schizosaccharomyces pombe* is distinct from its higher eukaryotic orthologs.
  • The conserved phosphatase family exhibits organism-specific functional diversification.