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Updated: May 6, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
07:23

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Published on: June 9, 2011

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Screening and identification of persistent viruses in cell lines derived from medically important arthropods

Ryo Matsumura1,2, Daisuke Kobayashi2,3, Astri Nur Faizah2

  • 1Graduate School of Agriculture, Meiji University, Kasawaki-shi, Kanagawa, Japan.

Journal of Medical Entomology
|February 28, 2024
PubMed

Insights

Researchers screened arthropod cell lines for persistent viral infections. They discovered 15 viruses, including 4 novel types, in mosquito and tick cells, impacting arbovirus research.

Area of Science:

  • Virology
  • Cell Biology
  • Entomology

Background:

  • Arthropod-derived cell lines are vital for studying arthropod-borne viruses (arboviruses).
  • Some cell lines harbor persistent, unapparent viral infections that can influence arbovirus replication.
  • Identifying these latent viruses is crucial for accurate arbovirus research.

Purpose of the Study:

  • To conduct a comprehensive virome analysis of various arthropod-derived cell lines.
  • To identify persistent viral infections within these cell lines.
  • To assess the presence of novel arthropod-specific viruses.

Main Methods:

  • Virome analysis of mosquito, tick, and flesh fly cell lines.
  • Detection and characterization of persistent viruses using molecular techniques.
  • Comparative analysis of viral loads across different cell lines.

Main Results:

  • Fifteen persistent viruses were detected in mosquito (NIID-CTR, Ar-3, MSQ43, NIAS-AeAl-2) and tick (IDE8) cell lines.
  • Eleven known arthropod-specific viruses and four novel viruses (Orthophasmavirus, Rhabdoviridae, Totiviridae, Bunyavirales) were identified.
  • Flesh fly (NIH-Sape-4) and one mosquito (CCL-126) cell lines appeared virus-free.

Conclusions:

  • Arthropod cell lines can harbor diverse persistent viral infections, including novel viral families.
  • The presence of these viruses can impact arbovirus studies, necessitating careful screening.
  • Further research is needed to understand the full impact of persistent viruses on arbovirus replication and experimental outcomes.
Keywords:
arthropod-specific viruscell linemosquitotickvirome

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