Cisgender cellular tissue-based products improve wound healing.

Area of Science:

• Regenerative Medicine
• Wound Healing Biology
• Tissue Engineering

Background:

• The sex-specific effects of cell-transfer products (CTP) on wound healing remain largely undescribed.
• Female CTP are derived from cryopreserved human placental membranes, while male CTP are bioengineered from human keratinocytes and foreskin fibroblasts.
• Understanding these differences is crucial for optimizing regenerative therapies.

Purpose of the Study:

• To investigate the influence of CTP sex on wound healing outcomes in both male and female recipients.
• To compare the efficacy of male and female CTP in promoting wound closure and tissue regeneration.
• To identify potential sex-based differences in the biological response to CTP.

Main Methods:

• Wound healing in a model system was examined to assess the role of CTP sex.
• Comparative analysis of wound closure rates and tissue regeneration metrics between male and female CTP groups.
• Evaluation of specific cellular and molecular markers associated with healing.

Main Results:

• Cisgender CTP wounds exhibited significantly better closure rates compared to allogeneic CTP.
• Male CTP demonstrated superior rates of cell proliferation (PC), endothelialization (PC-End), and collagen deposition (CC) over time compared to female CTP.
• Wound healing outcomes were influenced by factors including wound access, etiology, and duration of follow-up.

Conclusions:

• CTP sex plays a significant role in wound healing, with male CTP showing enhanced regenerative potential.
• The origin of CTP (placental vs. bioengineered) and recipient sex may influence therapeutic outcomes.
• Further research is warranted to elucidate the mechanisms underlying sex-specific CTP efficacy and to refine clinical applications.