Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.2K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.2K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

1.4K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
1.4K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CAMBRA caries risk stratification is associated with distinct salivary and supragingival plaque microbiomes in pre-orthodontic patients.

Scientific reports·2026
Same author

Direct powder extrusion 3D printing of poly(ɛ-caprolactone)-carbonate substituted hydroxyapatite powder composites: Influence of powder content and multi-scale spatial organization on scaffold morphology and mechanical properties.

Dental materials journal·2026
Same author

Enhanced Expansion of Bone Marrow-Derived Mesenchymal Stem Cells on Collagen-Coated Microcarriers Functionalized with Chimeric IGF-1-bFGF Proteins.

ACS omega·2026
Same author

Oral Microbiome Analysis and Caries Risk Classification Using the Caries Management by Risk Assessment System in Pre-Orthodontic Patients.

Journal of clinical medicine·2025
Same author

Enhanced Growth of Bone Marrow-Derived Mesenchymal Stem Cells on the Microcarriers Tethered With Engineered Basic Fibroblast Growth Factor.

Biotechnology journal·2025
Same author

Enhanced infiltration of myoblasts into collagen scaffolds loaded with engineered basic fibroblast growth factor.

Dental materials journal·2025

Related Experiment Video

Updated: Jul 2, 2025

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
07:20

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

Published on: February 28, 2020

9.9K

Structure and function of engineered stromal cell-derived factor-1α.

Fumika Abe1,2, Ayana Nakano1,2, Isao Hirata1

  • 1Department of Biomaterials, Graduate School of Biomedical and Health Sciences, Hiroshima University.

Dental Materials Journal
|February 28, 2024
PubMed
Summary

Chimeric proteins combining stromal cell-derived factor-1α (SDF) and collagen-binding domains (CBD) were synthesized for bone tissue engineering. Linking these domains impacts their function, but their order has minimal effect on the overall 3D structure.

Keywords:
Chimeric proteinScaffoldsStructural predictionTissue engineering

More Related Videos

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

12.4K
Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

3.4K

Related Experiment Videos

Last Updated: Jul 2, 2025

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery
07:20

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

Published on: February 28, 2020

9.9K
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
12:00

Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles

Published on: January 22, 2015

12.4K
Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
09:50

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Published on: August 20, 2021

3.4K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Protein Engineering

Background:

  • Collagen-based scaffolds are crucial for bone tissue engineering.
  • Stromal cell-derived factor-1α (SDF) and von Willebrand factor A3 collagen-binding domain (CBD) are key functional components.
  • Understanding domain linkage effects is vital for scaffold design.

Purpose of the Study:

  • To synthesize and characterize chimeric proteins of SDF and CBD for bone scaffolds.
  • To investigate the impact of domain linkage and order on protein structure and function.
  • To evaluate the potential of these chimeric proteins in tissue engineering applications.

Main Methods:

  • Synthesis of chimeric proteins comprising SDF and CBD.
  • Structural analysis using circular dichroism spectroscopy.
  • Functional assays including cell migration (SDF) and collagen-binding (CBD).
  • Computational structural prediction.

Main Results:

  • Chimeric protein synthesis was successful.
  • Linking SDF and CBD domains influenced their individual functions.
  • The order of domain linkage had minimal impact on the 3D structure of SDF and CBD.
  • Computational predictions aligned with experimental findings.

Conclusions:

  • Chimeric proteins can be engineered for bone tissue engineering scaffolds.
  • Domain linkage affects protein function, necessitating careful design.
  • The order of SDF and CBD domains has a limited effect on their structural integrity.
  • These findings provide insights for developing advanced biomaterials.