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Updated: Jul 1, 2025

Candidate Gene Testing in Clinical Cohort Studies with Multiplexed Genotyping and Mass Spectrometry
Published on: June 21, 2018
A combinatorial genetic strategy for exploring complex genotype-phenotype associations in cancer
Shan Li1, Alicia Wong1, Huiyun Sun1,2
1Human Biology Division, Fred Hutchinson Cancer Center, Seattle, WA, USA.
Abstract:
Available genetically defined cancer models are limited in genotypic and phenotypic complexity and underrepresent the heterogeneity of human cancer. Here, we describe a combinatorial genetic strategy applied to an organoid transformation assay to rapidly generate diverse, clinically relevant bladder and prostate cancer models. Importantly, the clonal architecture of the resultant tumors can be resolved using single-cell or spatially resolved next-generation sequencing to uncover polygenic drivers of cancer phenotypes.
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