Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

IgG Glycosylation Analysis in Patients with Ring14 Syndrome Unveils Novel Pathomechanisms and New Therapy Perspectives.

Biomolecules·2026

Same author

A splice-altering intronic variant in two multiplex families refines autosomal recessive COG4-related congenital disorder of glycosylation.

Molecular genetics and metabolism·2026

Same author

Lipooligosaccharide architecture in Acinetobacter modestus CM11G: a non-pathogenic strain.

Carbohydrate research·2025

Same author

The Other Side of the Same Coin: Beyond the Coding Region in Amyotrophic Lateral Sclerosis.

Pharmaceuticals (Basel, Switzerland)·2025

Same author

Tracking additives fate from a biodegradable mulch film to soil: a comprehensive LC-MS investigation.

Journal of hazardous materials·2025

Same author

Mono-allelic p.R37H Dehydrodolichyl Diphosphate Synthase variants lead to protein glycosylation defects, aberrant lipid profiles and interneuron scarcity in a novel mouse model of progressive epileptic encephalopathy.

bioRxiv : the preprint server for biology·2025

Same journal

Nanotechnology-Stem Cell Strategies in 3D Glioblastoma Organoid: Targeting Glioma Stem Cells Within a Complex Tumor Microenvironment.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of a Biosynthetic Gene Cluster by Capture Hi-C (CHi-C).

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Mapping the 3D Chromosome Organization of Streptomyces by Hi-C.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

CUT&Tag Epigenomic Profiling of Biosynthetic Gene Clusters in Arabidopsis thaliana.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Rhizobium rhizogenes-Mediated Hairy Root Transformation Protocol for Lotus japonicus and Other Legumes.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Characterization of Bioactive Saponins from Sea Cucumbers.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

CSF N-Glycomics Using MALDI MS Techniques.

Angela Messina¹, Donata Agata Romeo¹, Rita Barone^1,2

¹CNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.

Methods in Molecular Biology (Clifton, N.J.)

|March 1, 2024

Summary

This summary is machine-generated.

This study details a method for analyzing cerebrospinal fluid N-glycans, crucial for understanding Alzheimer's disease (AD). The optimized technique successfully linked specific N-glycan profiles to different AD stages.

Keywords:

AD CSF MALDI MS MCI N-glycans

More Related Videos

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

Related Experiment Videos

Last Updated: Jul 1, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

Area of Science:

Biochemistry
Analytical Chemistry
Neuroscience

Background:

Cerebrospinal fluid (CSF) N-glycans offer insights into neurological conditions.
Structural elucidation of N-glycans is vital for understanding their role in diseases like Alzheimer's disease (AD).

Purpose of the Study:

To present a robust methodology for the structural elucidation of the CSF N-glycome.
To optimize N-glycan analysis for high-quality mass spectrometry data.
To apply the developed method for identifying N-glycan profiles associated with Alzheimer's disease.

Main Methods:

N-glycans were released from glycoproteins using peptide-N-glycosidase F (PNGase F).
Purification involved C18 Sep-Pak and porous graphitized carbon (PGC) solid phase extraction (SPE).
Permethylation enhanced mass spectrometry sensitivity, followed by MALDI TOF MS and MS/MS analysis for molecular assignments.

Main Results:

Optimized methodology yields high-quality mass spectra with excellent signal-to-noise ratios up to m/z 4800.
The method successfully distinguished N-glycome profiles between early and advanced stages of Alzheimer's disease.

Conclusions:

The presented methodology provides a reliable approach for CSF N-glycome structural elucidation.
This technique is valuable for biomarker discovery in Alzheimer's disease and other neurological disorders.