[Vector plasmids and the strategy of molecular cloning for streptococci]

The experiments on elaboration of gene engineering methodology for streptococci are described. Two vectors were constructed for DNA cloning in streptococci on the basis of plasmids pSM19035 and pIP2501. Some of the plasmids occurred to be valuable vectors for molecular cloning in bacilli. Peculiarities of the transformation mechanism in streptococci were found to impede the molecular cloning. The recombination technique of cloning was successfully used in the streptococcal system.