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Engineered probiotics show promise for treating diseases linked to the human microbiome. Genetic tools enhance these live biotherapeutics, with prebiotics further improving their gut performance.

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Area of Science:

  • Microbiology
  • Biotechnology
  • Gastroenterology

Background:

  • Human microbiome dysbiosis is linked to various diseases, including inflammatory bowel disease, cancer, and obesity.
  • Next-generation probiotics, or live engineered organisms, represent an emerging therapeutic strategy.
  • The gut's harsh, nutrient-limited environment can hinder the efficacy of microbial therapies.

Purpose of the Study:

  • To review the current use of bacterial and yeast strains as probiotics.
  • To highlight advancements in genetic engineering for novel probiotic functions.
  • To report on therapeutic applications and clinical trials of engineered probiotics.

Main Methods:

  • Literature review of current probiotic applications and genetic engineering tools.
  • Analysis of recent therapeutic applications and clinical trial data for engineered probiotics.
  • Discussion of prebiotic supplementation strategies to enhance probiotic performance.

Main Results:

  • Bacterial and yeast strains are actively being developed and utilized as probiotics.
  • Genetic tools enable the engineering of enhanced therapeutic functions in microorganisms.
  • Engineered probiotics demonstrate potential in various therapeutic applications, with ongoing clinical investigations.

Conclusions:

  • Engineered probiotics offer a promising avenue for treating microbiome-associated diseases.
  • Advancements in genetic engineering are expanding the capabilities of live biotherapeutics.
  • Combining engineered probiotics with prebiotics may optimize therapeutic outcomes in the gut microbiome.