[Establishment and application of intrahepatic cholangiocarcinoma organoid models]

Intrahepatic cholangiocarcinoma(ICC) refers to cholangiocarcinomas originating from the secondary bile ducts within the liver and their branches.As a prevalent malignancy of the liver,the diagnosis and treatment of ICC pose significant challenges due to the high heterogeneity of the tumor and its propensity to develop drug resistance.Traditional drug screening and tumor mechanism studies have been confined to two-dimensional cell line cultures and patient-derived xenograft(PDX) models.However,cell lines cannot fully recapitulate the tumor heterogeneity,and PDX models have limitations such as high costs and time consumption,making them less practical for widespread clinical application.To address the limitations of these models,organoid models have been developed based on two-dimensional cell cultures.Organoid models combine the advantages of both aforementioned culture methods and offer unique strengths in cancer research.They provide a new perspective for studying the development and treatment of tumors. In this review, the focus is primarily on the latest advances in the field of organoids of ICC,with a particular emphasis on existing culture protocols and their potential applications in precision medicine and the establishment of biobanks.