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Updated: Jul 1, 2025
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A Rapid and Specific Microplate Assay for the Determination of Intra- and Extracellular Ascorbate in Cultured Cells
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[Improved Indophenol Titration Method for Ascorbic Acid Using a Dropper and Electronic Balance: Enhanced Convenience
Keizo Umegaki1,2, Mika Ito2, Kaori Yokotani2
1Center for Pharma-Food Research, Graduate School of Pharmaceutical Sciences, University of Shizuoka.
Summary
This study introduces a modified 2,6-dichloroindophenol (DCIP) titration for ascorbic acid analysis, replacing burettes with droppers and using an electronic balance. The new method is faster, uses fewer resources, and requires less skill for accurate vitamin C measurements.
Area of Science:
- Analytical Chemistry
- Biochemistry
Context:
- Ascorbic acid analysis is crucial for food and nutritional science.
- Traditional 2,6-dichloroindophenol (DCIP) titration requires specialized skills and equipment.
- Accurate vitamin C quantification is essential for quality control and health assessments.
Purpose:
- To develop a simplified and more accessible titration method for ascorbic acid quantification.
- To reduce the technical skill, time, and resource requirements of the DCIP titration method.
- To enhance the adaptability of ascorbic acid analysis for various sample concentrations.
Summary:
- A modified 2,6-dichloroindophenol (DCIP) titrimetric method for ascorbic acid analysis is presented, substituting a burette with a dropper and using an electronic balance for weight-based measurements.
- This approach significantly reduces analysis time by 43% and sample/reagent consumption by 50%, while lowering skill requirements.
- The method allows for flexible dropper selection and tailored DCIP standard solutions, improving convenience and adaptability, though endpoint determination challenges persist.
Impact:
- The modified method offers a more convenient and adaptable approach to ascorbic acid analysis, particularly beneficial for unskilled analysts.
- It enables quicker and more accurate vitamin C measurements in food samples.
- This innovation can broaden the accessibility of reliable ascorbic acid testing in various settings.
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