Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9.

bioRxiv : the preprint server for biology·2026

Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026

Same author

T-cell protrusions enable fast, localised initiation of chimeric antigen receptor signalling.

The EMBO journal·2026

Same author

Large-scale endoplasmic reticulum membrane solidification spatially organises proteins under thermal or metabolic stress.

bioRxiv : the preprint server for biology·2026

Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026

Same author

CDC42-Effector Proteins Regulate Higher Order Structure of Septins Required for CNS Myelin Integrity.

Glia·2026

Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026

Same journal

Distinct involvements of the subthalamic nucleus subpopulations in reward-biased decision-making in monkeys.

eLife·2026

Same journal

Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammation.

eLife·2026

Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026

Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026

Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jul 1, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

An open-source, high-resolution, automated fluorescence microscope.

Ando Christian Zehrer¹, Ana Martin-Villalba², Benedict Diederich³

¹Institut für Chemie und Biochemie, Freie Universität Berlin, Berlin, Germany.

|March 4, 2024

Summary

This summary is machine-generated.

Researchers developed an affordable, high-resolution fluorescence microscope using open-source hardware and software. This low-budget system enables high-quality imaging for labs and educational purposes, achieving single-molecule sensitivity.

Keywords:

HeLa cell biology cell culture human live cell

More Related Videos

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Related Experiment Videos

Last Updated: Jul 1, 2025

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy

Published on: January 18, 2017

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Application of High-speed Super-resolution SPEED Microscopy in Live Primary Cilium

Published on: January 16, 2018

Area of Science:

Biophysics
Microscopy
Open-source hardware

Background:

Sophisticated fluorescence microscopes are essential for life sciences but expensive, creating a bottleneck in data production.
Open-source hardware, leveraging 3D-printing and low-cost electronics, offers a potential solution.
Existing low-cost options often compromise image quality, while advanced designs require significant expertise.

Purpose of the Study:

To assemble an automated, high-resolution fluorescence microscope using open-source components and high-quality parts.
To demonstrate the system's capability for high-resolution imaging of both fixed and live samples.
To validate the system's performance for long-term live-cell imaging, single-molecule sensitivity, and super-resolution microscopy.

Main Methods:

Integration of the UC2 microscopy toolbox with high-quality components, electronics, and software.
Assembly of an automated, high-resolution fluorescence microscope.
Testing with fixed and live biological samples, including long-term incubations and super-resolution experiments.

Main Results:

Successful assembly of an automated, high-resolution fluorescence microscope.
Demonstration of high-resolution imaging for fixed and live samples, including multi-day live-cell imaging.
Achieved single-molecule sensitivity, enabling single particle tracking and SMLM super-resolution microscopy.

Conclusions:

A low-budget, open-source fluorescence microscope can achieve high image quality and advanced capabilities comparable to commercial systems.
The system empowers researchers with limited resources and serves as valuable educational material.
High-quality scientific data generation is feasible for users with minimal technical expertise using affordable, open-source tools.