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[Fluorescence confocal microscopy-complete digitization of pathology].
Andreas G Loth1, Anne Fassl2,3, Felix K H Chun2
1Universitätsklinikum Frankfurt, Klinik für Hals‑, Nasen- und Ohrenheilkunde, Goethe-Universität Frankfurt, Frankfurt am Main, Deutschland.
Pathologie (Heidelberg, Germany)
|March 6, 2024
Summary
Fluorescence-based confocal microscopy (FCM) creates virtual H&E sections, showing high agreement with conventional frozen sections. This technology could digitize pathology workflows, addressing specialist shortages in transplant pathology.
Area of Science:
- Pathology
- Microscopy
- Digital Health
Context:
- Fluorescence-based confocal microscopy (FCM) offers real-time virtual H&E section creation.
- Current applications include dermatopathology, uropathology, and gynecopathology.
- FCM enables fully digitized frozen sections, potentially replacing conventional methods.
Purpose:
- Implement FCM technology within digitized pathological workflows.
- Extend FCM use beyond liver transplant pathology to urology and otorhinolaryngology.
- Evaluate FCM performance against conventional frozen sections in liver transplant pathology.
Summary:
- FCM technique prospectively applied to donor liver tissue.
- Conventional frozen sections used for comparative analysis with virtual FCM scans.
- High agreement observed between FCM and conventional methods for detecting cholangitis, fibrosis, malignancy, steatosis, inflammation, steatohepatitis, and necrosis.
Impact:
- FCM can serve as a key component in achieving fully digitized pathology.
- Addresses challenges of specialist shortages and 24/7 frozen section availability in transplant pathology.
- Facilitates the extension of digital pathology to diverse disciplines like urology and otorhinolaryngology.
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