Related Experiment Video
Updated: Jul 1, 2025
Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells
Published on: August 26, 2021
Protocol to measure apoptosis-associated speck-like protein containing a CARD specks in human cerebrospinal fluid via
Kathryn E Sánchez1, Shanya Jiang2, Sharina Palencia Desai2
1Center for Memory and Aging, University of New Mexico, Albuquerque, NM 87131, USA; Department of Pediatrics, University of Chicago, Chicago, IL 60637, USA.
Abstract:
Apoptosis-associated speck-like protein containing a c-terminal caspase activation and recruitment domain (ASC) specks are elevated in the cerebrospinal fluid (CSF) of Alzheimer's disease and related dementias (AD/ADRDs) patients. Here, we present a flow cytometry protocol to quantify ASC specks. We describe steps for fluorescently labeling ASC specks using antibody technology, visualizing with imaging flow cytometry, and gating based on physical characteristics. CSF ASC specks levels positively correlate with phosphorylated tau (Thr181) and negatively correlate with amyloid β ratio (42/40), thus serving as a neuroinflammatory biomarker for diagnosing AD/ADRDs. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.
