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Updated: Jul 1, 2025
Fabrication of Three-dimensional Paper-based Microfluidic Devices for Immunoassays
Published on: March 9, 2017
Molecularly imprinted metal-organic frameworks assisted cloth and paper hybrid microfluidic devices for visual
Jiawen Xiang1, Ji Qi2, Die Hu3
1CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Research Centre for Coastal Environmental Engineering and Technology, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Abstract:
Marine algal toxin contamination is a major threat to human health. Thus, it is crucial to develop rapid and on-site techniques for detecting algal toxins. In this work, we developed colorimetric cloth and paper hybrid microfluidic devices (μCPADs) for rapid detection of gonyautoxin (GTX1/4) combined with molecularly imprinted polymers. In addition, the metal-organic frameworks (MOFs) composites were applied for this approach by their unique features. Guanosine serves as a dummy template for surface imprinting and has certain structural advantages in recognizing gonyautoxin. MOF@MIPs composites were able to perform a catalytic color reaction using hydrogen peroxide-tetramethylbenzidine for the detection of GTX1/4. The cloth-based sensing substrates were assembled on origami μPADs to form user-friendly, miniaturized colorimetric μCPADs. Combined with a smartphone, the proposed colorimetric μCPADs successfully achieved a low limit of detection of 0.65 μg/L within the range of 1-200 μg/L for rapid visual detection of GTX1/4. Moreover, the GTX1/4 of real shellfish and seawater samples were satisfactorily detected to indicate the application prospect of the μCPADs. The proposed method shows good potential in the low-cost, stable establishment of assays for the rapid detection of environmental biotoxins.
