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Related Experiment Video

Updated: Jul 1, 2025

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

Published on: May 17, 2010

Tissue-specific and endogenous protein labeling with split fluorescent proteins.

Gloria D Ligunas^1,2, German Paniagua¹, Jesselynn LaBelle^1,2

¹Department of Molecular Cell Biology, University of California, Merced, CA USA.

Biorxiv : the Preprint Server for Biology

|March 11, 2024

Summary

This summary is machine-generated.

Researchers developed a novel split fluorescent protein system for precise, tissue-specific protein labeling in zebrafish. This method overcomes limitations of current techniques, enabling detailed study of protein dynamics in whole organisms.

Keywords:

CRISPR-Cas Zebrafish protein tagging split fluorescent protein

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Last Updated: Jul 1, 2025

Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

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Published on: May 17, 2010

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

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Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

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Published on: December 24, 2016

Area of Science:

Molecular Biology
Genetics
Developmental Biology

Background:

Genetically encoded fluorescent proteins are vital for studying dynamic biological processes.
Existing methods for fluorescent protein fusion expression have limitations, particularly in whole organisms, including overexpression artifacts and technical difficulties with endogenous tagging.

Conclusions:

The split-mNG2 system provides a powerful tool for overcoming limitations in current protein labeling strategies.
This approach enables precise, tissue-specific, and endogenous protein labeling in zebrafish.
The system has broad applicability for diverse research areas in molecular and developmental biology.