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Updated: Jul 1, 2025
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Transplantation of a 3D Bioprinted Patch in a Murine Model of Myocardial Infarction
Published on: September 26, 2020
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In Situ Myocardial Regeneration With Tissue Engineered Cardiac Patch Using Spheroid-Based 3-Dimensional Tissue
Yojiro Koda1, Tatsuya Watanabe1, Keigo Kawaji2
1Department of Surgery, University of Chicago Medicine, Chicago, Illinois.
Annals of Thoracic Surgery Short Reports
|March 11, 2024
Summary
This study shows a new tissue-engineered cardiac patch promotes heart regeneration in pigs. The patch improved blood flow and electrical activity, demonstrating its potential for cardiac repair.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Cardiovascular Research
Background:
- Developing a novel tissue-engineered cardiac patch using 3D myocardial tissue and extracellular matrix to enhance in situ myocardial regeneration.
- Evaluating the patch's feasibility and effectiveness in a porcine model for both constructive and functional aspects of cardiac regeneration.
Purpose of the Study:
- To assess the potential of a novel tissue-engineered cardiac patch for promoting myocardial regeneration.
- To evaluate the constructive and functional outcomes of cardiac regeneration facilitated by the patch in a preclinical model.
Main Methods:
- Created a spheroid-based 3D multicellular tissue using cardiomyocytes and fibroblasts within a 3D net mold system.
- Incorporated extracellular matrix sheets and surgically implanted the construct into the right ventricle of healthy porcine models (n=4).
- Evaluated regeneration after 60 days using cardiac magnetic resonance imaging, electroanatomic mapping, and post-euthanasia mechanical viscoelasticity measurements.
Main Results:
- Cardiac MRI indicated improved regional tissue perfusion in the implanted patch area.
- Electroanatomic mapping demonstrated regenerated electrical conductivity within the patch, with preserved voltage regions (1.11 ± 0.8 mV).
- Histologic and tissue analyses confirmed host cell repopulation, including cardiomyocytes and vasculogenesis, supported by quantitative reverse transcription-polymerase chain reaction.
Conclusions:
- The tissue-engineered cardiac patch effectively promotes in situ constructive and functional myocardial regeneration.
- The patch demonstrated enhanced regional tissue perfusion and positive electrical activity in the porcine model, indicating successful cardiac repair.
