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Updated: Jul 1, 2025

Light-Controlled Fermentations for Microbial Chemical and Protein Production
Published on: March 22, 2022
Multimodal Control of Bacterial Gene Expression by Red and Blue Light
Stefanie S M Meier1, Elina Multamäki2, Américo T Ranzani1
1Department of Biochemistry, University of Bayreuth, Bayreuth, Germany.
None:
By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.
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