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4-Hydroxysesamin, a Modified Natural Compound, Attenuates Neuronal Apoptosis After Ischemic Stroke via Inhibiting
Lina Wang1, Zhenzhen Qu1, Qian Sun1
1Internal Medicine-Neurology, the Second Hospital of Hebei Medical University, Shijiazhuang, Hebei, People's Republic of China.
Neuropsychiatric Disease and Treatment
|March 12, 2024
Summary
This study shows that 4-hydroxysesamin (4-HS) protects neurons from injury and inflammation in preclinical models. 4-HS may work by influencing the MAPK pathway, offering potential neuroprotection.
Area of Science:
- Neuroscience
- Pharmacology
- Biochemistry
Background:
- 4-hydroxysesamin (4-HS) is a modified sesamin derivative.
- This study investigates the potential neuroprotective effects of 4-HS.
Purpose of the Study:
- To evaluate the neuroprotective properties of 4-HS in vitro and in vivo.
- To explore the underlying mechanisms, particularly the involvement of the MAPK pathway.
Main Methods:
- In vitro: HT22 cells exposed to oxygen-glucose deprivation and LPS, treated with 4-HS and/or metformin.
- In vivo: Middle cerebral artery occlusion (MCAO) stroke model in mice treated with 4-HS.
- Assays: CCK-8, flow cytometry, ELISA, Western blotting, histological staining.
Main Results:
- 4-HS demonstrated anti-inflammatory effects and improved cell viability in vitro.
- It reduced apoptosis and modulated key proteins in LPS-treated HT22 cells.
- In vivo, 4-HS improved outcomes in MCAO mice, reducing infarction and inflammation, and inhibited MAPK pathway phosphorylation.
Conclusions:
- 4-HS exhibits significant neuroprotective properties in both cellular and animal models.
- The neuroprotective effects are potentially mediated through the modulation of the MAPK signaling pathway.
