Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Tumor Microenvironment02:17

The Tumor Microenvironment

6.6K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

2.6K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
2.6K
Overview of Cell-Matrix Interactions01:24

Overview of Cell-Matrix Interactions

7.2K
The extracellular matrix or ECM holds cells together to form a tissue and allows the cells within the tissue to communicate. ECM comprises proteins such as fibronectin, collagen, laminin, etc. The most abundant protein in this space is collagen. Collagen fibers are interwoven with carbohydrate-containing protein molecules called proteoglycans. ECM allows cell migration and provides a structural scaffold at cell adhesion that anchors the cell when the extracellular matrix proteins interact with...
7.2K
Metastasis02:30

Metastasis

5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
Mechanism of Angiogenesis01:10

Mechanism of Angiogenesis

5.5K
Blood vessel formation starts early during embryonic development, around day 7. In the extraembryonic yolk sac, mesodermal precursor cells called hemangioblast proliferate and differentiate into angioblast. Angioblasts express vascular endothelial growth factor receptor 2 or VEGFR2, which binds VEGF-A, a proangiogenic factor, guiding blood vessel formation. VEGF signaling promotes angioblasts to form a blood island in the developing embryo. Angioblasts further differentiate, giving rise to...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Botryolides F and G from the Fungus <i>Bartalinia robillardoides</i> with Anthelmintic Activity.

Journal of natural products·2026
Same author

Family carer's experience of a delirium resource (PREDICT) to support care partnerships with healthcare professionals: A qualitative study.

Geriatric nursing (New York, N.Y.)·2026
Same author

Fibroblastic aspartoacylase suppresses TGFβ-mediated responses and cancer progression.

Nature communications·2026
Same author

Targeting RUNX1 protects against diastolic dysfunction in a two-hit mouse model of heart failure with preserved ejection fraction.

Cardiovascular research·2026
Same author

Increased mRNA translation delays tumour initiation and exposes a therapeutic vulnerability in lung cancer.

Molecular cancer·2026
Same author

E-cadherin inactivation shapes tumor microenvironment specificities in invasive lobular breast cancer.

Nature communications·2026

Related Experiment Video

Updated: Jul 1, 2025

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

7.5K

Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell function.

Alice Santi1,2, Emily J Kay1, Lisa J Neilson1

  • 1Cancer Research UK Scotland Institute, Glasgow G61 1BD, UK.

Science Signaling
|March 12, 2024
PubMed
Summary

Cancer-associated fibroblasts (CAFs) release extracellular vesicles (EVs) that transfer proteins to endothelial cells (ECs), influencing immune cell interactions. Matrix-bound EVs are key vehicles for this intercellular communication in solid tumors.

More Related Videos

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
06:35

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

4.5K
Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts
15:43

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

Published on: October 25, 2011

23.4K

Related Experiment Videos

Last Updated: Jul 1, 2025

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

7.5K
A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
06:35

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth

Published on: December 22, 2020

4.5K
Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts
15:43

Experimental Generation of Carcinoma-Associated Fibroblasts CAFs from Human Mammary Fibroblasts

Published on: October 25, 2011

23.4K

Area of Science:

  • Oncology
  • Cell Biology
  • Immunology

Background:

  • Intercellular communication is crucial for tumor progression and metastasis.
  • Cancer-associated fibroblasts (CAFs) significantly influence tumor microenvironment through their secretome.
  • Understanding CAF-secreted factors and their targets is vital for developing anti-cancer therapies.

Purpose of the Study:

  • To investigate the role of extracellular vesicles (EVs) released by CAFs in intercellular communication.
  • To identify proteins transferred from CAFs to endothelial cells (ECs) and their functional impact.
  • To elucidate the mechanism of protein transfer via matrix-bound EVs.

Main Methods:

  • Proteomic analysis of proteins transferred from human mammary CAFs to ECs.
  • Characterization of extracellular vesicles (EVs) produced by CAFs.
  • Functional assays to assess the impact of transferred proteins on cell adhesion.

Main Results:

  • CAFs release EVs containing plasma membrane receptors that are transferred to ECs.
  • Proteins transferred from CAFs, exemplified by THY1, enhance monocyte adhesion to ECs.
  • Matrix-bound EVs are identified as the primary mediators of protein transfer from CAFs.

Conclusions:

  • CAF-derived EVs mediate intercellular communication by transferring functional proteins to ECs.
  • This transfer influences immune cell interactions, potentially impacting tumor immunity.
  • CAF-derived matrix-bound EVs represent a novel therapeutic target for modulating tumor pathology.