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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Related Experiment Video

Updated: Jul 1, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Multifunctional

Zimu Tian¹, He Yan¹, Yong Zeng^1,2,3

¹Department of Chemistry, University of Florida, Gainesville, Florida 32611, United States.

ACS Applied Materials & Interfaces

|March 12, 2024

Summary

This study introduces a novel CRISPR-Cas12a assay (SPEEDi-CRISPR) for rapid, sensitive infectious disease diagnostics. It integrates target identification, enrichment, and signal generation, improving detection limits for point-of-care applications.

Keywords:

CRISPR/Cas12a HPV POCT detection magnetic beads

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

Area of Science:

Molecular Diagnostics
Biosensing Technology
CRISPR-based Systems

Background:

Resource-limited settings require effective diagnostic tools for infection management.
CRISPR-based biosensing offers next-generation diagnostics, including point-of-care (POC) testing.
Existing CRISPR diagnostics often require preamplification, limiting their efficiency.

Purpose of the Study:

To develop a novel CRISPR-Cas12a nucleic acid assay that integrates multiple functions.
To enhance diagnostic sensitivity and negate the need for preamplification.
To create a versatile platform for point-of-care infectious disease detection.

Main Methods:

Developed the Solid-Phase Extraction and Enhanced Detection Assay integrated by CRISPR-Cas12a (SPEEDi-CRISPR).
Utilized Cas12a-coated magnetic beads for solid-phase extraction, enrichment, and fluorogenic detection.
Integrated target identification, sequence-specific enrichment, and signal generation into a single platform.

Main Results:

Achieved detection limits from picomolar (pM) to femtomolar (fM) levels without preamplification.
Demonstrated high specificity, distinguishing HPV-18 from HPV-16 and Parvovirus B19.
Detected HPV-18 at concentrations as low as 2.3 fM (100 min) and 4.7 fM (60 min).
Successfully coupled the assay with smartphone-based fluorescence detection and lateral flow assays for POC applications.

Conclusions:

The SPEEDi-CRISPR assay offers a preamplification-free approach for highly sensitive nucleic acid detection.
This integrated platform leverages CRISPR-Cas12a's capabilities for efficient extraction, enrichment, and detection.
The assay shows promise for developing advanced, rapid, and accessible CRISPR diagnostics for infectious diseases.