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Related Concept Videos

Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution. In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
Titration of Polyprotic Base with a Strong Acid01:18

Titration of Polyprotic Base with a Strong Acid

The titration of a polyprotic base such as sodium carbonate with a strong acid such as hydrochloric acid results in two equivalence points on the titration curve. At the first equivalence point, the carbonate ions in the base are completely converted to bicarbonate ions. The second equivalence point corresponds to the complete conversion of bicarbonate ions to carbonic acid, which dissociates into carbon dioxide and water. The region before the first equivalence point corresponds to the...
Precipitation Titration: Endpoint Detection Methods01:19

Precipitation Titration: Endpoint Detection Methods

In argentometric precipitation titrations, endpoints can be detected visually by the Mohr, Volhard, and Fajans methods. In the Mohr method, adding a soluble chromate indicator gives an initial yellow color to the analyte solution. As the titrant is added, the first excess of silver ions forms a red silver chromate precipitate, marking the endpoint. The solution pH should be maintained at about 8 by adding solid CaCO3.
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EDTA: Indirect and Alkalimetric Titration01:23

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Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...

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Related Experiment Video

Updated: Jun 25, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
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A Fast and Simple Solid Phase Extraction-Based Method for Glucosinolate Determination: An Alternative to the ISO-9167

Yanfang Li1,2, Mengliang Zhang2, Pamela Pehrsson1

  • 1Methods and Application of Food Composition Laboratory, Beltsville Human Nutrition Research Center, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, MD 20705, USA.

Foods (Basel, Switzerland)
|March 13, 2024
PubMed
Summary
This summary is machine-generated.

A new method simplifies glucosinolate (GLS) analysis in Brassicaceae plants. This efficient technique offers improved accuracy and speed for quantifying these vital compounds, benefiting plant science and human health research.

Keywords:
Brassicaceae vegetablesISO 9167 methoddimethylaminopropyl (DEA)glucosinolatesquantificationsolid phase extraction (SPE) cartridgeweak anion exchange

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Area of Science:

  • Agricultural Chemistry
  • Plant Biochemistry
  • Analytical Chemistry

Background:

  • Glucosinolates (GLSs) are crucial sulfur-containing compounds in Brassicaceae plants, impacting plant defense and human health.
  • Accurate identification and quantification of total and individual GLS content are essential for research and quality control.

Purpose of the Study:

  • To develop an improved, efficient, and reliable method for glucosinolate extraction and quantification.
  • To provide an alternative to the existing ISO 9167-1 method for GLS analysis.

Main Methods:

  • Utilized a commercially available dimethylaminopropyl (DEA)-based weak anion exchange solid-phase extraction (SPE) cartridge for purification.
  • Employed ultra-high-performance liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for identification and quantification.

Main Results:

  • The developed method demonstrated comparable quantification of total and individual GLSs to the ISO method on certified rapeseeds and Brassicaceae vegetables.
  • The new SPE procedure is simpler, more efficient, and reduces analysis time.

Conclusions:

  • The improved SPE-UHPLC-HRMS method offers enhanced repeatability and accuracy for GLS analysis.
  • This method is suitable for large sample sizes, automation, and provides a valuable alternative to the traditional ISO method.