Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

7.9K
Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
7.9K
Ligand Binding and Linkage00:49

Ligand Binding and Linkage

4.8K
Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
4.8K
Ligand Binding Sites02:40

Ligand Binding Sites

12.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.8K
Metal-Ligand Bonds02:51

Metal-Ligand Bonds

20.8K
The hemoglobin in the blood, the chlorophyll in green plants, vitamin B-12, and the catalyst used in the manufacture of polyethylene all contain coordination compounds. Ions of the metals, especially the transition metals, are likely to form complexes.
In these complexes, transition metals form coordinate covalent bonds, a kind of Lewis acid-base interaction in which both of the electrons in the bond are contributed by a donor (Lewis base) to an electron acceptor (Lewis acid). The Lewis acid in...
20.8K
Stereoisomerism02:52

Stereoisomerism

11.9K
Isomerism in Complexes
Isomers are different chemical species that have the same chemical formula.
Transition metal complexes often exist as geometric isomers, in which the same atoms are connected through the same types of bonds but with differences in their orientation in space. Coordination complexes with two different ligands in the cis and trans positions from a ligand of interest form isomers. For example, the octahedral [Co(NH3)4Cl2]+ ion has two isomers (Figure 1) In the cis...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Luminescent Chiral Molecular Glasses by Melt-Quenching Enantiopure BINAP.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2025
Same author

Two RNA Folds from One Sequence: A Ribozyme with Versatile Substrate Processing Abilities.

Angewandte Chemie (International ed. in English)·2024
Same author

A pH-Responsive Topological Switch Based on a DNA Quadruplex-Duplex Hybrid.

Chemistry (Weinheim an der Bergstrasse, Germany)·2024
Same author

Impact of loop length and duplex extensions on the design of hybrid-type G-quadruplexes.

Chemical communications (Cambridge, England)·2023
Same author

Showcasing Different G-Quadruplex Folds of a G-Rich Sequence: Between Rule-Based Prediction and Butterfly Effect.

Journal of the American Chemical Society·2023
Same author

High-affinity binding at quadruplex-duplex junctions: rather the rule than the exception.

Nucleic acids research·2022

Related Experiment Video

Updated: Jul 1, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

611

Structural Differences at Quadruplex-Duplex Interfaces Enable Ligand-Induced Topological Transitions.

Yoanes Maria Vianney1, Dorothea Dierks1, Klaus Weisz1

  • 1Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Str. 4, D-17489, Greifswald, Germany.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|March 13, 2024
PubMed
Summary
This summary is machine-generated.

This study reveals how quadruplex-duplex (QD) junctions bind ligands. A specific human telomeric QD hybrid structure shifts its fold upon Phen-DC3 binding, demonstrating ligand-induced structural changes.

Keywords:
NMR spectroscopyPhen‐DC3induced fitintercalationquadruplex‐duplex junction

More Related Videos

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K

Related Experiment Videos

Last Updated: Jul 1, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

611
Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers
08:28

Single-molecule Manipulation of G-quadruplexes by Magnetic Tweezers

Published on: September 19, 2017

8.0K
Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping
05:32

Author Spotlight: Characterizing DNA G-Quadruplex by Bis-3-Chloropiperidine Based Chemical Mapping

Published on: May 12, 2023

1.3K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biophysics

Background:

  • Quadruplex-duplex (QD) junctions are significant structural motifs in biology and technology.
  • These junctions serve as high-affinity binding sites for various ligands.
  • Understanding QD junction structures is crucial for developing novel therapeutic and diagnostic tools.

Purpose of the Study:

  • To structurally characterize a human telomeric QD hybrid construct using Nuclear Magnetic Resonance (NMR).
  • To investigate the binding mechanism and structural transition induced by the ligand Phen-DC3.
  • To elucidate the molecular basis for ligand discrimination of QD junctions.

Main Methods:

  • Nuclear Magnetic Resonance (NMR) spectroscopy was employed for structural characterization.
  • The study involved analyzing a human telomeric QD hybrid construct in potassium buffer.
  • Ligand binding studies with Phen-DC3 were performed to observe structural changes.

Main Results:

  • The QD hybrid construct exists as a mixture of (3+1) hybrid and chair-type (2+2) antiparallel quadruplex species.
  • A unique capping structure, a T·AH+·G·C quartet, stabilizes the antiparallel species.
  • Phen-DC3 binding induces a topological transition, favoring the exclusive formation of the (3+1) hybrid fold.

Conclusions:

  • The study provides unprecedented insight into the discrimination of QD junctions by Phen-DC3.
  • The observed ligand-induced structural transition follows an induced fit mechanism.
  • This work advances the understanding of QD junction-ligand interactions and their potential applications.