Related Experiment Video
Updated: Jul 1, 2025

10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
12.0K
N-terminal proteomics of Mycobacterium marinum using bottom-up label-free quantitative analysis in data-dependent
Daniel D Hu1, Simon D Weaver1, Owen A Collars2
1Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana, USA.
Microbiology Resource Announcements
|March 13, 2024
Abstract:
N-terminal acetylation in Mycobacterium tuberculosis is correlated with pathogenic activity. We used genomics and bottom-up proteomics to identify protein Emp1 as the sole acetyltransferase responsible for acetylation of EsxA, a known virulence factor. Using custom data analysis, we screened the proteome to identify 22 additional putative substrates of Emp1.

