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Updated: Jul 1, 2025

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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Semirational Design Based on Consensus Sequences to Balance the Enzyme Activity-Stability Trade-Off

Yang Zhao1,2, Kun Chen1, Haixia Yang1

  • 1National Engineering Research Center for Fruit & Vegetable Processing, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Non-Thermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Journal of Agricultural and Food Chemistry
|March 13, 2024
PubMed
Summary

Enzyme engineering overcomes the stability-activity trade-off. Novel mutations in Rosa roxburghii copper-zinc superoxide dismutase (RrCuZnSOD) enhanced both stability and activity, offering new possibilities for enzyme applications.

Keywords:
consensus sequencesmolecular dynamics simulationprotein engineeringstability-activity trade-offthermostability

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