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Related Concept Videos

Transcription Factors02:16

Transcription Factors

75.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
75.8K
General Transcription Factors01:30

General Transcription Factors

5.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.3K
Transducer Mechanism: Nuclear Receptors01:31

Transducer Mechanism: Nuclear Receptors

1.3K
Nuclear receptors, or NRs, are unique transcription factors that regulate gene transcription and affect the cellular pathways involved in reproduction, development, or metabolism. Their ability to be stimulated by small lipophilic ligands and control vital cellular processes makes them ideal drug targets. Nearly 10-15% of currently prescribed drugs target these receptors.
About 48 different soluble family members of nuclear receptors are identified that can be divided into two main classes:
1.3K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

5.7K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
5.7K
Nuclear Export01:42

Nuclear Export

3.6K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
3.6K
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

4.6K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
4.6K

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Updated: Jul 1, 2025

A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry
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A Simple and Efficient Method to Detect Nuclear Factor Activation in Human Neutrophils by Flow Cytometry

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Nuclear Transcription Factor Detection.

Joseph A Straub1, Jamie J Newman2

  • 1Department of Biology and Microbiology, Northwestern State University, Natchitoches, LA, USA.

Methods in Molecular Biology (Clifton, N.J.)
|March 13, 2024
PubMed
Summary
This summary is machine-generated.

This study details methods for measuring transcription factors in human fat cells at both the RNA and protein levels. These protocols ensure accurate analysis of gene expression and cell characterization.

Keywords:
Adipose stromal/stem cellsGene expressionProtein expressionTranscription factors

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Stem Cell Research

Background:

  • Cellular fate is regulated by nuclear transcription factors.
  • Accurate detection of these factors is crucial for understanding cell function.

Purpose of the Study:

  • To establish comprehensive protocols for detecting transcription factors in human adipose cells.
  • To enable reliable assessment of transcription factor expression at both transcript and protein levels.

Main Methods:

  • Transcript analysis: RNA extraction, reverse transcription polymerase chain reaction (RT-PCR), endpoint PCR, and quantitative RT-PCR (RT-qPCR).
  • Protein analysis: Protein extraction, Bradford assay, SDS-PAGE, western blotting, and ImageJ quantification.

Main Results:

  • Detailed step-by-step protocols are provided for each method.
  • These methods allow for the reliable detection and quantification of transcription factors.

Conclusions:

  • The described protocols are essential for reproducible transcription factor assessment.
  • These techniques aid in the characterization of human adult-derived adipose stromal/stem cells.