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Related Concept Videos

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Related Experiment Video

Updated: Jul 1, 2025

Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
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A novel optimized pre-embedding antibody-labelling correlative light electron microscopy technique.

Nicole Doyle1, Jennifer Simpson1, Philippa C Hawes1,2

  • 1The Pirbright Institute, Ash Road, Pirbright, Woking, GU24 0NF, UK.

Access Microbiology
|March 14, 2024
PubMed
Summary
This summary is machine-generated.

This study adapts correlative light electron microscopy (CLEM) for antibody labeling, enabling cellular structure localization. The method is useful for labs without tagged proteins or specialized CLEM equipment.

Keywords:
CLEMCoVantibody CLEMconfocal microscopycoronaviruscorrelativeelectron microscopyreplication organelle

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Area of Science:

  • Cell biology
  • Microscopy techniques
  • Molecular imaging

Background:

  • Correlative light electron microscopy (CLEM) is crucial for high-resolution cellular event localization.
  • Traditional CLEM often relies on tagged proteins or viruses for fluorescence correlation.
  • Dedicated CLEM instruments are not universally available in research laboratories.

Purpose of the Study:

  • To adapt CLEM for antibody labeling in cellular studies.
  • To assess the feasibility and utility of antibody-based CLEM.
  • To provide an alternative CLEM approach for researchers.

Main Methods:

  • Adaptation of correlative light electron microscopy (CLEM) protocols.
  • Utilizing antibody labeling for fluorescence correlation.
  • Investigating the sensitivity of cellular structures to the adapted CLEM process.

Main Results:

  • Successful adaptation of CLEM for antibody labeling.
  • Identification of cellular structure sensitivity variations during the process.
  • Demonstration of the technique's applicability in standard laboratory settings.

Conclusions:

  • Antibody-based CLEM is a viable alternative to traditional methods.
  • The technique offers valuable insights into cellular structures.
  • This approach expands CLEM accessibility for broader research applications.