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PacBio HiFi reads data sets for the ATCC-authenticated isolate of Candida albicans SC5314 (ATCC MYA-2876)
Lois L Hoyer1, Elizabeth K Hogan2, Alvaro G Hernandez2
1Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Access Microbiology
|August 14, 2026
Summary
New high-quality DNA sequencing data for the medically important yeast Candida albicans (SC5314 isolate) were generated using PacBio HiFi technology. This valuable resource aids in exploring diploid loci and improving the Candida albicans genome assembly.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Candida albicans is a significant human pathogen.
- The existing genome sequence of C. albicans SC5314 is a phased diploid assembly.
- Advancements in DNA sequencing offer opportunities to enhance genome resources.
Purpose of the Study:
- To generate high-quality Pacific Biosciences (PacBio) HiFi sequencing data for Candida albicans SC5314.
- To assess the utility of this data for constructing an improved phased diploid genome assembly.
- To provide a valuable resource for further research on C. albicans.
Main Methods:
- Sequencing of the C. albicans SC5314 isolate using PacBio HiFi technology.
- Generation of long reads with high accuracy and depth.
- Bioinformatic analysis to evaluate genome assembly potential.
Main Results:
- PacBio HiFi data exhibited exceptional read length (mean >14 kb) and depth.
- Most chromosomes were resolved as gapless, phased haplotypes.
- Challenging regions, such as the rDNA locus, required further investigation for full resolution.
Conclusions:
- The generated PacBio HiFi data represent a significant advancement for C. albicans genomics.
- These data facilitate the construction of a more complete and accurate phased diploid genome assembly.
- The data are publicly available and will aid in exploring diploid loci in this key reference strain.
