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Related Experiment Video

Updated: May 24, 2025

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

Published on: March 10, 2023

Deciphering age-related transcriptomic changes in the mouse retinal pigment epithelium.

Sushil K Dubey¹, Rashmi Dubey¹, Kyungsik Jung¹

¹Department of Surgery, East Tennessee State University, Johnson City, TN 37614, USA.

|March 5, 2025

Summary

Aging retinal pigment epithelium (RPE) shows increased inflammation and oxidative stress. An in vitro model of aged human RPE mimics these changes, aiding research into age-related retinal diseases.

Keywords:

chronological aging inflammation oxidative stress retinal pigment epithelium transcriptome

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Last Updated: May 24, 2025

A Protocol to Evaluate and Quantify Retinal Pigmented Epithelium Pathologies in Mouse Models of Age-Related Macular Degeneration

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Published on: March 10, 2023

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Published on: December 22, 2014

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Efficient Dissection and Culture of Primary Mouse Retinal Pigment Epithelial Cells

Published on: February 10, 2021

Area of Science:

Ophthalmology
Cellular Biology
Gerontology

Background:

Aging retinal pigment epithelium (RPE) homeostasis declines, impacting retinal health.
Understanding RPE aging mechanisms is key to age-related retinal pathologies.
Research on RPE aging is limited by a lack of in vitro models.

Purpose of the Study:

Compare transcriptomes of young and aged mouse RPE.
Evaluate an in vitro model of chronologically aged primary human RPE.
Investigate RPE aging mechanisms and screen therapeutic compounds.

Main Methods:

Transcriptome comparison between young and aged mouse RPE.
Evaluation of an in vitro model using chronologically aged primary human RPE.
Gene expression profiling of the in vitro model.

Main Results:

Aging mouse RPE showed upregulated immunogenic, proinflammatory, and oxidative stress genes.
Aging RPE exhibited dysregulated visual perception and extracellular matrix pathways.
The in vitro human RPE model displayed gene expression patterns similar to native-aged RPE.

Conclusions:

Aging RPE is characterized by inflammation, immune activation, and oxidative stress.
The in vitro model is a valuable tool for studying RPE aging.
Findings provide insights into age-related increases in retinal pathology risk.