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Updated: Jan 8, 2026

Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
Published on: August 12, 2019
A telomere-to-telomere genome assembly for Meyerozyma guilliermondii ATCC 6260: closing gaps and resolving a
Lois L Hoyer1, Brian A Freeman1, Elizabeth K Hogan2
1Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Abstract:
Meyerozyma guilliermondii, a haploid yeast (phylum Ascomycota), is useful in bioprocessing and bioremediation, and is an infrequent pathogen of humans. The availability of highly accurate, long-read DNA sequencing methods provided the opportunity to improve the M. guilliermondii genome assembly. Using Pacific Biosciences (PacBio) HiFi technology, we generated a chromosome-level, telomere-to-telomere assembly for the M. guilliermondii type strain ATCC 6260. This assembly closed gaps in the current reference sequence and resolved a translocation artefact that affected the size and structure of chromosomes 4 and 5. The improved genome sequence is available publicly and will facilitate future studies of this species.
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