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PacBio HiFi reads data sets for the ATCC-authenticated isolate of Candida albicans SC5314 (ATCC MYA-2876)
Lois L Hoyer1, Elizabeth K Hogan2, Alvaro G Hernandez2
1Department of Pathobiology, College of Veterinary Medicine, University of Illinois Urbana-Champaign, Urbana, IL, USA.
Abstract:
Candida albicans is a yeast of major medical importance. The genome sequence for the reference isolate SC5314 has been available for many years and is displayed as a phased diploid assembly through a public community resource. As DNA sequencing technologies continue to advance, new high-quality data sets can be generated to augment existing genome resources. Here, we present Pacific Biosciences (PacBio) HiFi data sets with exceptional read length and sequencing depth. The data sets were generated from C. albicans SC5314 newly obtained from the American Type Culture Collection, thereby authenticating the isolate to a community-accessible source. The PacBio HiFi data set with a mean read length greater than 14 kb was assessed for its potential to support construction of a phased diploid genome assembly. Most chromosomes were effortlessly resolved as gapless, phased haplotypes with telomeres at both ends; however, challenging regions including the rDNA locus on one copy of chromosome R remained longer than could be fully resolved using these high-quality, state-of-the-art data. The PacBio HiFi reads data sets are available through GenBank and provide a valuable resource for exploring diploid loci in this important reference strain.
Insights
New high-quality DNA sequencing data for the medically important yeast Candida albicans (SC5314 isolate) were generated using PacBio HiFi technology. This valuable resource aids in exploring diploid loci and improving the Candida albicans genome assembly.
Area of Science:
- Microbiology
- Genomics
- Molecular Biology
Background:
- Candida albicans is a significant human pathogen.
- The existing genome sequence of C. albicans SC5314 is a phased diploid assembly.
- Advancements in DNA sequencing offer opportunities to enhance genome resources.
Purpose of the Study:
- To generate high-quality Pacific Biosciences (PacBio) HiFi sequencing data for Candida albicans SC5314.
- To assess the utility of this data for constructing an improved phased diploid genome assembly.
- To provide a valuable resource for further research on C. albicans.
Main Methods:
- Sequencing of the C. albicans SC5314 isolate using PacBio HiFi technology.
- Generation of long reads with high accuracy and depth.
- Bioinformatic analysis to evaluate genome assembly potential.
Main Results:
- PacBio HiFi data exhibited exceptional read length (mean >14 kb) and depth.
- Most chromosomes were resolved as gapless, phased haplotypes.
- Challenging regions, such as the rDNA locus, required further investigation for full resolution.
Conclusions:
- The generated PacBio HiFi data represent a significant advancement for C. albicans genomics.
- These data facilitate the construction of a more complete and accurate phased diploid genome assembly.
- The data are publicly available and will aid in exploring diploid loci in this key reference strain.
