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Two-Dimensional Visualization and Quantification of Labile, Inorganic Plant Nutrients and Contaminants in Soil

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In Vitro Fossilization for High Spatial Resolution Quantification of Elements in Plant-Tissue Using LA-ICP-TOFMS.

Pascal Becker¹, Thomas Nauser¹, Matthias Wiggenhauser²

¹Laboratory of Inorganic Chemistry, Department of Chemistry and Applied Biosciences, ETH Zurich, Zurich 8093, Switzerland.

Analytical Chemistry

|March 14, 2024

Summary

This summary is machine-generated.

Fossilizing plant tissues creates a mineral-like matrix for accurate elemental mapping using laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS). This method ensures reliable quantification and high-resolution elemental images in crops.

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Area of Science:

Analytical Chemistry
Plant Science
Geochemistry

Background:

Quantitative elemental mapping in biological tissues using laser ablation inductively coupled plasma time-of-flight mass spectrometry (LA-ICP-TOFMS) is challenging due to complex matrix effects.
Conventional sample preparation for LA-ICP-TOFMS in biological samples can lead to irregular ablation and misleading elemental quantification.
Existing methods struggle with accurate, high-resolution elemental analysis of plant tissues.

Purpose of the Study:

To develop and validate a novel sample preparation method for quantitative elemental mapping of plant tissues using LA-ICP-TOFMS.
To overcome the challenges of biotissue matrix effects and irregular ablation in elemental quantification.
To enable high-resolution, reproducible elemental imaging of crop samples.

Main Methods:

Plant tissues (sunflower, soybean, corn leaves) were fossilized to create a stable, mineral-like matrix.
Silicone was used as an internal standard for normalization during LA-ICP-TOFMS analysis.
Quantitative elemental maps were generated and validated against inductively coupled plasma - optical emission spectrometry (ICP-OES) analysis.

Main Results:

The fossilization method enabled consistent ablation, reducing image blurring and improving quantification accuracy.
Accurate, high-resolution elemental maps of cadmium distribution in crop leaves were successfully generated.
Results demonstrated that fossilization corrects misleading trends caused by irregular ablation in conventionally dried samples.

Conclusions:

Fossilization is a feasible and effective sample preparation technique for quantitative elemental mapping of plant tissues using LA-ICP-TOFMS.
This method allows for reproducible, high-resolution elemental imaging without extensive sample preparation.
The approach is suitable for analyzing various plant samples and has implications for agricultural soil monitoring and plant physiology studies.