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Updated: Jun 30, 2025
00:07
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
Published on: August 23, 2019
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CircNRCAM up-regulates NRCAM to promote papillary thyroid carcinoma progression
1Department of Thyroid Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1 East Jianshe Road, Zhengzhou, 450052, Henan, China.
Journal of Endocrinological Investigation
|March 15, 2024
Summary
Circular RNA NRCAM (circNRCAM) promotes papillary thyroid carcinoma (PTC) progression by down-regulating miR-506-3p, leading to increased Neural Cell Adhesion Molecule (NRCAM) expression and enhanced cell proliferation and migration.
Area of Science:
- Endocrinology
- Oncology
- Molecular Biology
Background:
- Papillary Thyroid Carcinoma (PTC) is the most common thyroid cancer.
- Neural Cell Adhesion Molecule (NRCAM) is known to be upregulated in PTC and promotes cancer cell growth and movement.
- The precise mechanism of NRCAM's role in PTC remains unclear.
Purpose of the Study:
- To elucidate the underlying mechanism of NRCAM in papillary thyroid carcinoma (PTC) cells.
- To identify potential therapeutic targets for PTC treatment.
Main Methods:
- Bioinformatics analysis
- Western blot
- Colony formation assays
- Dual-luciferase reporter assays
- Loss-of-function experiments
Main Results:
- NRCAM and circNRCAM were overexpressed in PTC tissues and cells.
- circNRCAM positively regulates NRCAM expression.
- Both circNRCAM and NRCAM promote PTC cell proliferation, invasion, and migration.
- circNRCAM competitively binds to ALKBH5, inhibiting miR-506-3p and upregulating NRCAM.
Conclusions:
- circNRCAM enhances PTC cell biological behaviors by upregulating NRCAM via downregulating miR-506-3p.
- This pathway presents a potential therapeutic strategy for PTC.
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