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Updated: May 5, 2026
Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
Published on: February 7, 2018
Precisely modulating the chromatin tracker via substituent engineering: reporting pathological oxidative stress
Jinsong Li1, Yingyong Ni1, Junjun Wang1
1School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, Institute of Physical Science and Information Technology, Center of Free Electron Laser & High Magnetic Field, Key Laboratory of Structure and Functional Regulation of Hybrid Materials, Ministry of Education, Key Laboratory of Functional Inorganic Materials Chemistry, Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University Hefei 230601 P. R. China zhpzhp@263.net wangjunjun@ahu.edu.cn.
Abstract:
An in-depth understanding of cancer-cell mitosis presents unprecedented advantages for solving metastasis and proliferation of tumors, which has aroused great interest in visualizing the behavior via a luminescence tool. We developed a fluorescent molecule CBTZ-yne based on substituent engineering to acquire befitting lipophilicity and electrophilicity for anchoring lipid droplets and the nucleus, in which the low polarity environment and nucleic acids triggered a "weak-strong" fluorescence and "short-long" fluorescence-lifetime response. Meaningfully, CBTZ-yne visualized chromatin condensation, alignment, pull-push, and separation as well as lipid droplet dynamics, for the first time, precisely unveiling the asynchronous cellular mitosis processes affected by photo-generation reactive oxygen species according to the subtle change of fluorescence-lifetime. Our work suggested a new guideline for tracking the issue of the proliferation of malignant tumors in photodynamic therapy.
