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Updated: Jun 30, 2025
The Chick Chorioallantoic Membrane CAM Model as a Tool to Study Ovarian Tissue Transplantation
Published on: June 23, 2023
Protocol for xenografting of BxPC-3 pancreatic tumors using a chorioallantoic membrane model
Robin Colenbier1, Lenny Coppens1, Tine Logghe2
1Laboratory of Cell Biology and Histology, Faculty of Medicine and Health Sciences, Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, 2610 Antwerp, Belgium.
Abstract:
The chorioallantoic membrane (CAM) model is an increasingly attractive model for the study of human tumors. However, concise techniques for the use of pancreatic ductal adenocarcinoma BxPC-3 xenografts in CAM assays are not yet available. Here, we present a protocol for the induction of BxPC-3 xenograft tumors with high grafting efficiency. We describe steps for embryo incubation, egg handling, and grafting, each of which has been optimized to prevent fungal contamination and minimize mortality.
