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Updated: Jun 30, 2025
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Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
Published on: December 1, 2016
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Quantitative Superresolution Imaging of F-Actin in the Cell Body and Cytoskeletal Protrusions Using Phalloidin-Based
Hirushi Gunasekara1, Thilini Perera1, Chih-Jia Chao2
1Department of Chemistry, College of Liberal Arts and Sciences, University of Illinois Chicago, Chicago, IL, 60607, USA.
Biorxiv : the Preprint Server for Biology
|March 18, 2024
Summary
We developed a new super-resolution microscopy technique using dye-conjugated phalloidin for clearer imaging of filamentous actin (F-actin). This method improves cellular structure preservation and actin quantification at the nanoscale.
Area of Science:
- Cell Biology
- Microscopy
- Biophysics
Background:
- Filamentous actin (F-actin) is crucial for cellular structure and dynamics.
- Super-resolution microscopy techniques are needed for nanoscale F-actin visualization.
- Existing methods can struggle with sample preservation and consistent quantification.
Conclusions:
- Phalloidin-based SMLLM offers a robust method for nanoscale F-actin imaging with superior structural preservation and quantification.
- This technique provides a valuable tool for studying dynamic F-actin rearrangements in response to stimuli.
- The developed SMLLM approach has potential for broader applications in protein-specific single-molecule imaging.
